HSP90 alpha peptide - 380-0P

Heat shock proteins 90 (HSP90) are molecular chaperones

control peptide

Cat. No.: 380-0P

Amount: 100 µg

Price: $105.00

Cat. No. 380-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 701 to 717 from human HSP90 α (UniProt Id: P07900)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	380 003
Remarks	This control peptide consists of the synthetic peptide (aa 701-717 of human HSP 90α) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	380-0p.pdf

Cat. No.: 380-0P

Amount: 100 µg

Price: $105.00

Background

Heat shock proteins 90 (HSP 90) are molecular chaperones. Two isoforms (α and β) have been described so far. They promote the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. They undergo a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation.
HSP 90s interact dynamically with various co-chaperones that modulate their substrate recognition, ATPase cycle and chaperone function.

1 ) Batista FA et al., Curr. Protein Pept. Sci. (2015)
2 ) Khurana N et al., Front Oncol (2015)
3 ) Richter K et al., Nat. Struct. Mol. Biol. (2007)
4 ) Minami Y et al., J. Biol. Chem. (1991)
5 ) Gruppi CM et al., Mol. Reprod. Dev. (1991)
6 ) Howard KJ et al., J. Biol. Chem. (1990)
7 ) Koyasu S et al., Proc. Natl. Acad. Sci. U.S.A. (1986)

Protocols

OTHER

Preadsorbtion

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