HSP90 alpha antibody - 380 003 K.O.

Heat shock proteins 90 (HSP90) are molecular chaperones

Rabbit polyclonal purified antibody

Cat. No.: 380 003

Amount: 50 µg

Price: $375.00

Cat. No. 380 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 701 to 717 from human HSP90 α (UniProt Id: P07900)
Reactivity	Reacts with: human (P07900), rat (P82995), mouse (P07901). Other species not tested yet.
Specificity	K.O. validated PubMed: 31849607
Matching control protein/peptide	380-0P
Data sheet	380_003.pdf

References for HSP90 alpha - 380 003

The Alpha Isoform of Heat Shock Protein 90 and the Co-chaperones p23 and Cdc37 Promote Opioid Anti-nociception in the Brain.
Lei W, Duron DI, Stine C, Mishra S, Blagg BSJ, Streicher JM
Frontiers in molecular neuroscience (2019) 12: 294. 380 003 IHC; KO verified; tested species: mouse

Cat. No.: 380 003

Amount: 50 µg

Price: $375.00

ICC

IHC-P

Background

Heat shock proteins 90 (HSP 90) are molecular chaperones. Two isoforms (α and β) have been described so far. They promote the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. They undergo a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation.
HSP 90s interact dynamically with various co-chaperones that modulate their substrate recognition, ATPase cycle and chaperone function.

1 ) Batista FA et al., Curr. Protein Pept. Sci. (2015)
2 ) Khurana N et al., Front Oncol (2015)
3 ) Richter K et al., Nat. Struct. Mol. Biol. (2007)
4 ) Minami Y et al., J. Biol. Chem. (1991)
5 ) Gruppi CM et al., Mol. Reprod. Dev. (1991)
6 ) Howard KJ et al., J. Biol. Chem. (1990)
7 ) Koyasu S et al., Proc. Natl. Acad. Sci. U.S.A. (1986)

Protocols

ICC

IHC-P

OTHER

Preabsorbtion

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