Synaptic Systems - Homer1b/c

Cat. No.: 160 023

Amount: 50 µg

Price: $465.00

Cat. No. 160 023	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to the c-terminal half of human Homer 1b (UniProt Id: Q86YM7-1)
Reactivity	Reacts with: human (Q86YM7-1), rat (Q9Z214-2, Q9Z214-1), mouse. Other species not tested yet.
Specificity	Specific for homer 1b and 1c; no cross-reactivity to homer 1a.
Matching control protein/peptide	160-02P
Data sheet	160_023.pdf

References for Homer1b/c - 160 023

A single 24 h maternal separation at PND 9 promotes behavioral resilience of female C57BL/6J mice and the possible role of hippocampal Homer1a.
Hao Y, Niu Y, Shi F, Zhang L, Peng C, Yan Z, Chen X, Xu H
Heliyon (2024) 105: e27037. 160 023 WB; tested species: mouse

Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 023 WB, EM; tested species: rat

Dysregulated postsynaptic density and endocytic zone in the amygdala of human heroin and cocaine abusers.
Okvist A, Fagergren P, Whittard J, Garcia-Osta A, Drakenberg K, Horvath MC, Schmidt CJ, Keller E, Bannon MJ, Hurd YL
Biological psychiatry (2011) 693: 245-52. 160 023 WB; tested species: human

Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways.
Roselli F, Hutzler P, Wegerich Y, Livrea P, Almeida OF
PloS one (2009) 46: e6011. 160 023 WB; tested species: rat

Axonal plasticity in response to active forces generated through magnetic nano-pulling.
Falconieri A, De Vincentiis S, Cappello V, Convertino D, Das R, Ghignoli S, Figoli S, Luin S, Català-Castro F, Marchetti L, Borello U, et al.
Cell reports (2022) 421: 111912. 160 023 ICC; tested species: mouse

Kainate Receptor Activation Shapes Short-Term Synaptic Plasticity by Controlling Receptor Lateral Mobility at Glutamatergic Synapses.
Polenghi A, Nieus T, Guazzi S, Gorostiza P, Petrini EM, Barberis A
Cell reports (2020) 3110: 107735. 160 023 ICC; tested species: mouse

Preparation of dissociated mouse primary neuronal cultures from long-term cryopreserved brain tissue.
Cano-Jaimez M, Tagliatti E, Mendonca P, Nicholson E, Vivekananda U, Kullmann DM, Volynski KE
Journal of neuroscience methods (2019) : 108452. 160 023 ICC; tested species: mouse

NMDA-induced accumulation of Shank at the postsynaptic density is mediated by CaMKII.
Tao-Cheng JH, Yang Y, Bayer KU, Reese TS, Dosemeci A
Biochemical and biophysical research communications (2014) 4501: 808-11. 160 023 ICC

Nova proteins direct synaptic integration of somatostatin interneurons through activity-dependent alternative splicing.
Ibrahim LA, Wamsley B, Alghamdi N, Yusuf N, Sevier E, Hairston A, Sherer M, Jaglin XH, Xu Q, Guo L, Khodadadi-Jamayran A, et al.
eLife (2023) 12: . 160 023 IHC; tested species: mouse

Loss of miR-183/96 alters synaptic strength via pre- and postsynaptic mechanisms at a central synapse.
Krohs C, Körber C, Ebbers L, Altaf F, Hollje G, Hoppe S, Dörflinger Y, Prosser HM, Nothwang HG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 160 023 IHC; tested species: mouse

GABA-receptive microglia selectively sculpt developing inhibitory circuits.
Favuzzi E, Huang S, Saldi GA, Binan L, Ibrahim LA, Fernández-Otero M, Cao Y, Zeine A, Sefah A, Zheng K, Xu Q, et al.
Cell (2021) 18415: 4048-4063.e32. 160 023 IHC; tested species: mouse

Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS.
Bączyk M, Alami NO, Delestrée N, Martinot C, Tang L, Commisso B, Bayer D, Doisne N, Frankel W, Manuel M, Roselli F, et al.
The Journal of experimental medicine (2020) 2178: . 160 023 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 160 023 IHC; tested species: rat

Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus.
Heise C, Schroeder JC, Schoen M, Halbedl S, Reim D, Woelfle S, Kreutz MR, Schmeisser MJ, Boeckers TM
Frontiers in cellular neuroscience (2016) 10: 106. 160 023 IHC

Cat. No.: 160 023

Amount: 50 µg

Price: $465.00

ICC

IHC

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)
6 ) Ciruela F et al., Mol. Cell. Neurosci. (2000)
7 ) Xiao B et al., Curr. Opin. Neurobiol. (2000)
8 ) Tu JC et al., Neuron (1999)
9 ) Brakeman PR et al., Nature (1997)
10 ) Shiraishi Y et al., J. Comp. Neurol. (2004)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Homer1b/c antibody - 160 023

References for Homer1b/c - 160 023