Synaptic Systems - Glycine transporter2

Cat. No.: 272 011

Amount: 100 µg

Price: $415.00

Molecular and functional profiling of cell diversity and identity in the lateral superior olive, an auditory brainstem center with ascending and descending projections.
Maraslioglu-Sperber A, Pizzi E, Fisch JO, Kattler K, Ritter T, Friauf E
Frontiers in cellular neuroscience (2024) 18: 1354520. 272 011 IHC; tested species: mouse

WB

ICC

IHC

IHC-P

Background

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.

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2 ) James VM et al., Biol. Chem. (2012)
3 ) Tsai G et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
4 ) Gomeza J et al., Neuron (2003)
5 ) Geerlings A et al., J. Biol. Chem. (2001)
6 ) Martínez-Maza R et al., J. Biol. Chem. (2001)
7 ) Zafra F et al., J. Neurosci. (1995)
8 ) Adams RH et al., J. Neurosci. (1995)
9 ) Jursky F et al., J. Neurochem. (1995)

Protocols

WB

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 272 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: yes gallery IHC: 1 : 250 up to 1 : 500 gallery IHC-P: 1 : 100 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	117F12
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity	Reacts with: mouse (Q761V0), rat (P58295). Other species not tested yet.
Matching control protein/peptide	272-0P
Remarks	WB: Glycine transporter 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	272_011.pdf

Glycine transporter2 antibody - 272 011

References for Glycine transporter2 - 272 011