Synaptic Systems - Glycine transporter2

Cat. No.: 272 006

Amount: 50 µg

Price: $380.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

IHC

IHC-P

Background

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.

1 ) Kurolap A et al., Am. J. Hum. Genet. (2016)
2 ) James VM et al., Biol. Chem. (2012)
3 ) Tsai G et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
4 ) Gomeza J et al., Neuron (2003)
5 ) Geerlings A et al., J. Biol. Chem. (2001)
6 ) Martínez-Maza R et al., J. Biol. Chem. (2001)
7 ) Zafra F et al., J. Neurosci. (1995)
8 ) Adams RH et al., J. Neurosci. (1995)
9 ) Jursky F et al., J. Neurochem. (1995)

Protocols

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 272 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 1:500 up to 1 : 1:1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity	Reacts with: rat (P58295), mouse (Q761V0). Other species not tested yet.
Matching control protein/peptide	272-0P
Data sheet	272_006.pdf

Glycine transporter2 antibody - 272 006