Synaptic Systems - Glycine transporter2

Cat. No.: 272 004

Amount: 100 µl

Price: $365.00

Cat. No. 272 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity	Reacts with: rat (P58295), mouse (Q761V0). Other species not tested yet.
Specificity	K.D. validated PubMed: 30881475
Matching control protein/peptide	272-0P
Remarks	WB: Glycine transporter 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	272_004.pdf

References for Glycine transporter2 - 272 004

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 272 004 ICC; tested species: rat

Multimodal sensory control of motor performance by glycinergic interneurons of the mouse spinal cord deep dorsal horn.
Gradwell MA, Ozeri-Engelhard N, Eisdorfer JT, Laflamme OD, Gonzalez M, Upadhyay A, Medlock L, Shrier T, Patel KR, Aoki A, Gandhi M, et al.
Neuron (2024) 1128: 1302-1327.e13. 272 004 IHC; tested species: mouse

Characterization of three cholinergic inputs to the cochlear nucleus.
Beebe NL, Herrera YN, Noftz WA, Roberts MT, Schofield BR
Journal of chemical neuroanatomy (2023) 131: 102284. 272 004 IHC; tested species: mouse

Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 272 004 IHC, EM; tested species: mouse

Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 272 004 IHC; tested species: rat

Group I metabotropic glutamate receptor-triggered temporally patterned action potential-dependent spontaneous synaptic transmission in mouse MNTB neurons.
Wang H, Peng K, Curry RJ, Li D, Wang Y, Wang X, Lu Y
Hearing research (2023) 435: 108822. 272 004 IHC; tested species: mouse

Neurotransmitter phenotype and axonal projection patterns of VIP-expressing neurons in the inferior colliculus.
Beebe NL, Silveira MA, Goyer D, Noftz WA, Roberts MT, Schofield BR
Journal of chemical neuroanatomy (2022) 126: 102189. 272 004 IHC; tested species: mouse

Morphological and neurochemical characterization of glycinergic neurons in laminae I-IV of the mouse spinal dorsal horn.
Miranda CO, Hegedüs K, Wildner H, Zeilhofer HU, Antal M
The Journal of comparative neurology (2021) : . 272 004 IHC; tested species: mouse

Cholinergic boutons are closely associated with excitatory cells and four subtypes of inhibitory cells in the inferior colliculus.
Beebe NL, Schofield BR
Journal of chemical neuroanatomy (2021) 116: 101998. 272 004 IHC; tested species: mouse

Opposing effects of an atypical glycinergic and substance P transmission on interpeduncular nucleus plasticity.
Melani R, Von Itter R, Jing D, Koppensteiner P, Ninan I
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2019) : . 272 004 IHC; tested species: mouse

Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse.
Tulloch AJ, Teo S, Carvajal BV, Tessier-Lavigne M, Jaworski A
The Journal of comparative neurology (2019) 52718: 2948-2972. 272 004 IHC; tested species: mouse

Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.
Bai F, Ma Y, Guo H, Li Y, Xu F, Zhang M, Dong H, Deng J, Xiong L
Evidence-based complementary and alternative medicine : eCAM (2019) 2019: 7493286. 272 004 IHC-P; KD verified; tested species: rat

Cat. No.: 272 004

Amount: 100 µl

Price: $365.00

ICC

IHC

IHC-P

Background

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.

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2 ) James VM et al., Biol. Chem. (2012)
3 ) Tsai G et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
4 ) Gomeza J et al., Neuron (2003)
5 ) Geerlings A et al., J. Biol. Chem. (2001)
6 ) Martínez-Maza R et al., J. Biol. Chem. (2001)
7 ) Zafra F et al., J. Neurosci. (1995)
8 ) Adams RH et al., J. Neurosci. (1995)
9 ) Jursky F et al., J. Neurochem. (1995)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Glycine transporter2 antibody - 272 004 K.D.

References for Glycine transporter2 - 272 004