Synaptic Systems - Glycine transporter2

Cat. No.: 272 003

Amount: 50 µg

Price: $380.00

Cat. No. 272 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: yes gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Clarity: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Clarity is a sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. Clearing is carried out by SDS and Focusclear treatment. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity	Reacts with: rat (P58295), mouse (Q761V0). Other species not tested yet.
Specificity	K.D. validated PubMed: 30881475
Matching control protein/peptide	272-0P
Remarks	WB: Glycine transporter 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	272_003.pdf

References for Glycine transporter2 - 272 003

Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.
Bai F, Ma Y, Guo H, Li Y, Xu F, Zhang M, Dong H, Deng J, Xiong L
Evidence-based complementary and alternative medicine : eCAM (2019) 2019: 7493286. 272 003 WB; KD verified; tested species: rat

Sequential maturation of stimulus-specific adaptation in the mouse lemniscal auditory system.
Valerio P, Rechenmann J, Joshi S, De Franceschi G, Barkat TR
Science advances (2024) 101: eadi7624. 272 003 IHC; tested species: mouse

Kv3 channels contribute to the excitability of sub-populations of spinal cord neurons in lamina VII.
Mullen P, Pilati N, Large CH, Deuchars J, Deuchars S
eNeuro (2022) : . 272 003 IHC; tested species: mouse

Structural arrangement of auditory brainstem nuclei in the bats Phyllostomus discolor and Carollia perspicillata.
Pätz C, Console-Meyer L, Felmy F
The Journal of comparative neurology (2022) : . 272 003 IHC

CX3CR1 mutation alters synaptic and astrocytic protein expression, topographic gradients, and response latencies in the auditory brainstem.
Milinkeviciute G, Chokr SM, Castro EM, Cramer KS
The Journal of comparative neurology (2021) 52911: 3076-3097. 272 003 IHC; tested species: mouse

In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.
Tai W, Wu W, Wang LL, Ni H, Chen C, Yang J, Zang T, Zou Y, Xu XM, Zhang CL
Cell stem cell (2021) 285: 923-937.e4. 272 003 IHC; tested species: mouse

Arrangement of excitatory synaptic inputs on dendrites of the medial superior olive.
Callan AR, Heß M, Felmy F, Leibold C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 272 003 IHC

Slow NMDA-mediated excitation accelerates offset-response latencies generated via a post-inhibitory rebound mechanism.
Rajaram E, Kaltenbach C, Fischl MJ, Mrowka L, Alexandrova O, Grothe B, Hennig MH, Kopp-Scheinpflug C
eNeuro (2019) : . 272 003 IHC; tested species: mouse

Hyper-formation of GABA and glycine co-releasing terminals in the mouse cerebellar nuclei after deprivation of GABAergic inputs from Purkinje cells.
Kobayashi S, Kim J, Yanagawa Y, Suzuki N, Saito H, Takayama C
Neuroscience (2019) : . 272 003 IHC, EM; tested species: mouse

Distinct Distribution Patterns of Potassium Channel Sub-Units in Somato-Dendritic Compartments of Neurons of the Medial Superior Olive.
Nabel AL, Callan AR, Gleiss SA, Kladisios N, Leibold C, Felmy F
Frontiers in cellular neuroscience (2019) 13: 38. 272 003 IHC

Tonotopic alterations in inhibitory input to the medial nucleus of the trapezoid body in a mouse model of Fragile X syndrome.
McCullagh EA, Salcedo E, Huntsman MM, Klug A
The Journal of comparative neurology (2017) 52516: 3543-3562. 272 003 IHC; tested species: mouse

Physiology and anatomy of neurons in the medial superior olive of the mouse.
Fischl MJ, Burger RM, Schmidt-Pauly M, Alexandrova O, Sinclair JL, Grothe B, Forsythe ID, Kopp-Scheinpflug C
Journal of neurophysiology (2016) 1166: 2676-2688. 272 003 IHC; tested species: mouse

Aging alters mechanisms underlying voluntary movements in spinal motor neurons of mice, primates, and humans.
Castro RW, Lopes MC, Settlage RE, Valdez G
JCI insight (2023) 89: . 272 003 Clarity; tested species: mouse

Cat. No.: 272 003

Amount: 50 µg

Price: $380.00

ICC

IHC

IHC-P

Background

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.

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2 ) James VM et al., Biol. Chem. (2012)
3 ) Tsai G et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
4 ) Gomeza J et al., Neuron (2003)
5 ) Geerlings A et al., J. Biol. Chem. (2001)
6 ) Martínez-Maza R et al., J. Biol. Chem. (2001)
7 ) Zafra F et al., J. Neurosci. (1995)
8 ) Adams RH et al., J. Neurosci. (1995)
9 ) Jursky F et al., J. Neurochem. (1995)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Glycine transporter2 antibody - 272 003 K.D.

References for Glycine transporter2 - 272 003