Glycine receptor antibody - 146 011BT

An inhibitory receptor in the nervous system

Mouse monoclonal purified IgG

Cat. No.: 146 011BT

Amount: 100 µg

Price: $465.00

Cat. No. 146 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) IP: yes ICC: yes IHC: 1 : 250 up to 1 : 500 (see remarks) gallery IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	biotin
Clone	mAb4a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop	AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity	Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish. Other species not tested yet.
Specificity	Specific for all glycine receptor subunits.
Remarks	IHC: Tissue sections require additional antigen retrieval with 95% methanol, 5% acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001). ELISA: The ELISA-protocol for membrane proteins is recommended.
Data sheet	146_011bt.pdf

Cat. No.: 146 011BT

Amount: 100 µg

Price: $465.00

IHC

Background

The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.

1 ) Bechade C et al., Eur. J. Neurosci. (1996)
2 ) Becker CM et al., J. Neurosci. (1986)
3 ) Altschuler RA et al., Brain Res. (1986)
4 ) Triller A et al., J. Cell Biol. (1985)
5 ) Graham D et al., Biochemistry (1985)

Protocols

Standard protocol

ICC

IHC

ELISA

Membrane proteins

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