Cat. No.: 146 011BT
Amount: 100 µg
Price:
$465.00
Cat. No. 146 011BT |
100 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: yes Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 250 up to 1 : 500 (see remarks) gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.', $event)" style="cursor: help;">ELISA: yes (see remarks) |
Label | biotin |
Clone | mAb4a |
Subtype | IgG1 (κ light chain) |
Immunogen | Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727) |
Epitop |
AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727) |
Reactivity |
Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish. Other species not tested yet. |
Specificity | Specific for all glycine receptor subunits. |
Remarks |
IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001). |
Data sheet | 146_011bt.pdf |