Cat. No.: 198 104
Amount: 100 µl
Price:
$375.00
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| Cat. No. 198 104 |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320) |
| Reactivity |
Reacts with: human (Q99259), rat (P18088), mouse (P48318). Other species not tested yet. |
| Specificity | Specific for GAD 2 / GAD 65. |
| Matching control protein/peptide | 198-1P |
| Remarks |
WB: The Guinea pig antiserum is less sensitive than the rabbit antiserum (cat. no. 198 102). |
| Data sheet | 198_104.pdf |
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The glutamic acid decarboxylases GAD1 and GAD2, also referred to as GAD67, and GAD65 respectively, synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. Therefore, GADs are widely used markers for the GABAergic system (1). The hydrophilic GAD1 can heterodimerize with the membrane achored GAD2 and a part of GAD1 is targeted to inhibitory nerve terminals by this mechanism (2). Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule (3).
GAD1 and 2 also occur in rodent pancreatic islets of Langerhans, whereas human islets mainly express GAD2 which has been identified as a major autoantigen in type 1 diabetes (3).