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Endophilin1 peptide - 159-0P

Endophilin1 is an SH3 domain protein involved in clathrin mediated endocytosis
control peptide
Cat. No.: 159-0P
Amount: 100 µg
Price: $105.00
Cat. No. 159-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
Immunogen Synthetic peptide corresponding to AA 256 to 276 from mouse Endophilin1 (UniProt Id: Q62420)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 159 002, 159 004
Remarks

This control peptide consists of the synthetic peptide (QPKPRMSLEFATGDSTQ) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 159-0p.pdf
Cat. No.: 159-0P
Amount: 100 µg
Price: $105.00
Background

Endophilins are SH3 domain proteins involved in endocytosis. Both, Endophilin 1 and 2 have been shown to play important roles in clathrin mediated synaptic vesicle recycling. They recruite and stabilize the polyphosphoinositide phosphatase synaptojanin at nerve terminals.

The divergent C-terminal tail of VgluT1 has been reported to be a binding partner of Endophilin A1.

In contrast to Endophilin 1 that shows a brain specific expression, Endophilin 2 is abundantly expressed in different tissues.

Protocols