Synaptic Systems - Dynamin3

Cat. No.: 115-3P

Amount: 100 µg

Price: $105.00

Background

Dynamin was discovered because of its binding to microtubules. It was later shown not to function in the cytoskeleton but in endocytosis. Dynamin is required for clathrin - mediated endocytosis. It contains a NH2 - terminal GTPase domain, a middle pleckstrin - homology domain, and a COOH- terminal proline - rich sequence. The COOH - terminal sequence binds to amphiphilin which contains a SH3 domain that recognizes the proline - rich sequence of dynamin.
There are at least three isoforms of dynamin: Dynamin 1 is enriched in synapses whereas dynamin 2 is ubiquitous and dynamin 3 is expressed in brain and testis. Neuronal dynamin 1 is phosphorylated by protein kinase C and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is possible that the dephosphorylation provides a trigger for endocytosis.

1 ) Hinshaw JE, Annual review of cell and developmental biology (2000) 16: 483-519.
2 ) Jahn R, Annual review of neuroscience (1994) 17: 219-46.
3 ) Slepnev VI, Nature reviews. Neuroscience (2000) 13: 161-72.
4 ) Brodin L, Current opinion in neurobiology (2000) 103: 312-20.
5 ) Hannah MJ, Annual review of cell and developmental biology (1999) 15: 733-98.

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 115-3P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 773 to 794 from mouse Dynamin3 (UniProt Id: Q8BZ98)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	115 302, 115 303
Remarks	This control peptide consists of the synthetic peptide (aa 773 - 794 in mouse dynamin 3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	115-3p.pdf

Dynamin3 peptide - 115-3P