Synaptic Systems - beta-Catenin

Cat. No.: 281 004

Amount: 100 µl

Price: $365.00

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 281 004 IHC; tested species: rat

WB

ICC

IHC

Background

α, β and γ-catenin are intracellular proteins that link cadherins to the actin cytosceleton. Cadherins are cell-surface proteins that are involved in cell-cell adhesion.
α-N-catenin is expressed mainly in the nervous system. It is a cytoplasmic protein that interacts with N-cadherin and functions in cell-cell adhesion. It is a regulator for the stability of synaptic contacts and is important for cerebellar and hippocampal lamination. There are two isoforms which are differentially expressed during development.
The major part of β-catenin localizes to the cell membrane and is part of E-cadherin/catenin adhesion complexes.

1 ) Taylor AM et al., J. Neurosci. (2013)
2 ) Wu H et al., Development (2012)
3 ) Nandadasa S et al., PLoS ONE (2012)
4 ) Kumamoto N et al., Nat. Neurosci. (2012)
5 ) Abe K et al., Nat. Neurosci. (2004)
6 ) Park C et al., Nat. Genet. (2002)
7 ) Benson DL et al., J. Neurosci. (1998)
8 ) Shibuya Y et al., J. Neurocytol. (1996)
9 ) Knudsen KA et al., J. Cell Biol. (1995)
10 ) Hinck L et al., J. Cell Biol. (1994)

Protocols

WB

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 281 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 768 to 782 from mouse β-Catenin (UniProt Id: Q02248)
Reactivity	Reacts with: rat (Q9WU82), mouse (Q02248). Other species not tested yet.
Specificity	Specific for β-catenin
Matching control protein/peptide	281-0P
Data sheet	281_004.pdf

beta-Catenin antibody - 281 004

References for beta-Catenin - 281 004