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beta-Catenin - 281-0P

Cadherin binding partners
control peptide
Cat. No.: 281-0P
Amount: 100 µg
Price: $90.00
Cat. No. 281-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Immunogen Synthetic peptide corresponding to AA 768 to 782 from mouse β-Catenin (UniProt Id: Q02248)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 281 003, 281 004
Remarks

This control peptide consists of the synthetic peptide (aa 768 - 782 of mouse β-catenin) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 281-0p.pdf
Cat. No.: 281-0P
Quantity: 100 µg
Price: $90.00
Background
α, β and γ-catenin are intracellular proteins that link cadherins to the actin cytosceleton. Cadherins are cell-surface proteins that are involved in cell-cell adhesion.
α-N-catenin is expressed mainly in the nervous system. It is a cytoplasmic protein that interacts with N-cadherin and functions in cell-cell adhesion. It is a regulator for the stability of synaptic contacts and is important for cerebellar and hippocampal lamination. There are two isoforms which are differentially expressed during development.
The major part of β-catenin localizes to the cell membrane and is part of E-cadherin/catenin adhesion complexes.
Protocols