Caspase3 antibody - 236 003

Caspase3 is a key regulator of apoptosis

Rabbit polyclonal purified antibody

Cat. No.: 236 003

Amount: 50 µg

Price: $375.00

Cat. No. 236 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 170 to 175 from mouse Caspase3 (UniProt Id: P70677)
Reactivity	Reacts with: human (P42574), rat (P55213), mouse (P70677), cow. Other species not tested yet.
Specificity	Recognizes cleaved and activated caspase 3.
Data sheet	236_003.pdf

Cat. No.: 236 003

Amount: 50 µg

Price: $375.00

ICC

Background

Cell proliferation, differentiation and apoptosis are central features of tissue homeostasis. Inhibition of apoptosis might be involved in the pathogenesis of cancer by extending cell life and growth.
The activation of caspases, particularly caspase 3, appears to be a central part in most apoptotic pathways. The inactive caspase 3 protoenzyme is activated by proteolytic cleavage behind aspartic acid at position 175 leading to activated p17 and p12 fragments. In its active form caspase 3 cleaves many key proteins like the nuclear enzyme poly (ADPribose) polymerase (PARP).

1 ) Sunyach C et al., J. Biol. Chem. (2007)
2 ) Zhang YX et al., Acta Pharmacol. Sin. (2005)
3 ) Tang D et al., J. Biol. Chem. (2000)
4 ) Nicholson DW et al., Nature (1995)
5 ) Fernandes-Alnemri T et al., J. Biol. Chem. (1994)

Protocols

ICC