Calcineurin A protein - 387-0P

Calcineurin is a calcium/calmodulin dependent Ser/Thr protein phosphatase

control protein

Cat. No.: 387-0P

Amount: 100 µg

Price: $105.00

Cat. No. 387-0P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 1 to 521 from human Calcineurin (UniProt Id: Q08209)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	387 002, 387 003
Remarks	This control protein consists of the recombinant protein (full length human Calcineurin A) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	387-0p.pdf

Cat. No.: 387-0P

Amount: 100 µg

Price: $105.00

Background

Calcineurin (PP2B) is a calcium/calmodulin dependent Ser/Thr protein phosphatase that is expressed at high levels in the CNS. It is a heterodimeric protein consisting of a catalytic subunit calcineurin A containing an active site dinuclear metal center, and a tightly associated, myristoylated, Ca²⁺-binding subunit called calcineurin B. Calcineurin is involved in many cellular processes and Ca²⁺-dependent signal transduction pathways.

1 ) Celsi F et al., Neurobiol. Dis. (2007)
2 ) Rusnak F et al., Physiol. Rev. (2000)
3 ) Mulkey RM et al., Nature (1994)
4 ) Liu J et al., Cell (1991)
5 ) Klee CB et al., Proc. Natl. Acad. Sci. U.S.A. (1979)

Protocols

OTHER

Preadsorbtion

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