Synaptic Systems - beta3-Integrin

Cat. No.: 240 303

Amount: 50 µg

Price: $375.00

WB

Background

Integrins are heterodimers consisting of noncovalently associated α and β subunits. More than 20 different integrin receptors composed of 16 different α and 8 different β subunits have been described so far. Most of these receptors bind components of the extracellular matrix like fibronectin, collagen and vitronectin.
Integrins are transmembrane glycoproteins involved in many normal cellular processes (embryogenesis, hemostasis, tissue repair, immune response) but also in abnormal pathological events like metastatic spread of tumor cells. In humans five isoforms of β1-integrin have been described (β1A-1D, 1C-2). β1-integrin is also known as very late antigen VLA or CD29.
β3-integrin, also referred to a CD 61, is found with the alpha IIb chain in platelets.

1 ) Dingemans AM et al., Mol. Cancer (2010)
2 ) Liu S et al., Arthritis Rheum. (2009)
3 ) Loulier K et al., PLoS Biol. (2009)
4 ) Shi F et al., J. Cell. Sci. (2008)
5 ) Young-Pearse TL et al., Neural Dev (2008)
6 ) Nodari A et al., J. Cell Biol. (2007)
7 ) Benninger Y et al., J. Neurosci. (2006)
8 ) Tiwari-Woodruff SK et al., J. Cell Biol. (2001)
9 ) Grabham PW et al., J. Neurosci. (1997)
10 ) Sato T et al., Blood (1997)

Protocols

WB

OTHER

Preabsorbtion

Other products for this target

Cat. No. 240 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 76 to 91 from mouse β3-Integrin (UniProt Id: O54890)
Reactivity	Reacts with: rat (Q8R2H2), mouse (O54890). Other species not tested yet.
Specificity	Specific for β3-integrin.
Matching control protein/peptide	240-3P
Data sheet	240_303.pdf

beta3-Integrin antibody - 240 303