Synaptic Systems - beta3-Integrin

Cat. No.: 240-3P

Amount: 100 µg

Price: $105.00

Background

Integrins are heterodimers consisting of noncovalently associated α and β subunits. More than 20 different integrin receptors composed of 16 different α and 8 different β subunits have been described so far. Most of these receptors bind components of the extracellular matrix like fibronectin, collagen and vitronectin.
Integrins are transmembrane glycoproteins involved in many normal cellular processes (embryogenesis, hemostasis, tissue repair, immune response) but also in abnormal pathological events like metastatic spread of tumor cells. In humans five isoforms of β1-integrin have been described (β1A-1D, 1C-2). β1-integrin is also known as very late antigen VLA or CD29.
β3-integrin, also referred to a CD 61, is found with the alpha IIb chain in platelets.

1 ) Dingemans AM et al., Mol. Cancer (2010)
2 ) Liu S et al., Arthritis Rheum. (2009)
3 ) Loulier K et al., PLoS Biol. (2009)
4 ) Shi F et al., J. Cell. Sci. (2008)
5 ) Young-Pearse TL et al., Neural Dev (2008)
6 ) Nodari A et al., J. Cell Biol. (2007)
7 ) Benninger Y et al., J. Neurosci. (2006)
8 ) Tiwari-Woodruff SK et al., J. Cell Biol. (2001)
9 ) Grabham PW et al., J. Neurosci. (1997)
10 ) Sato T et al., Blood (1997)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 240-3P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 76 to 91 from mouse β3-Integrin (UniProt Id: O54890)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	240 303
Remarks	This control peptide consists of the synthetic peptide (aa 76 - 91 of mouse β3-integrin) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	240-3p.pdf

beta3-Integrin peptide - 240-3P