Synaptic Systems - alpha-Internexin

Cat. No.: 167 002

Amount: 200 µl

Price: $355.00

WB

ICC

Background

The cytoskeleton of most eukaryotic cells is composed of three distinct components: Actin-based microfilaments, tubulin based microtubules and intermediate filaments (IFs).
α-Internexin is a neuronal intermediate filament of type four. It is assumed to be expressed by all neurons and preceeds the onset of the expression of the heavy medium and light variants of neurofilaments which are major components of the neuronal IFs.
Alterations in the phosphorylation state of IFs have been associated with neurodegenerative diseases like Alzheimer, Parkinson, dementia with Lewy bodies (DLB), and motor neuron disease (MND).

1 ) Yuan A et al., J. Neurosci. (2006)
2 ) Armstrong RA et al., Eur. J. Neurol. (2006)
3 ) Ko TL et al., J. Biomed. Sci. (2005)
4 ) Chien CL et al., J. Neurosci. Res. (2005)
5 ) Levavasseur F et al., Brain Res. Mol. Brain Res. (1999)
6 ) Ching GY et al., J. Neurosci. (1999)
7 ) Abumuhor IA et al., J. Struct. Biol. (1998)
8 ) Suzuki T et al., Brain Res. (1997)
9 ) Benson DL et al., J. Neurocytol. (1996)
10 ) Tanaka J et al., Biochem. Biophys. Res. Commun. (1993)

Protocols

WB

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 167 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 499 from human α-Internexin (UniProt Id: Q16352)
Reactivity	Reacts with: rat (P23565), mouse (P46660). Other species not tested yet.
Specificity	Specific for α-internexin.
Matching control protein/peptide	167-0P
Remarks	ICC: Ice cold methanol fixation is recommended.
Data sheet	167_002.pdf

alpha-Internexin antibody - 167 002