Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Spinophilin antibody - 399 003 K.O.

Spinophilin is enriched at dendritic spines
Rabbit polyclonal purified antibody
Cat. No.: 399 003
Amount: 50 µg
Price: $375.00
Cat. No. 399 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
DNA-PAINT: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 290 to 302 from rat Spinophilin (UniProt Id: O35274)
Reactivity Reacts with: rat (O35274), mouse (Q6R891).
Other species not tested yet.
Specificity Specific for Spinophilin isoform1. Does not detect isoform 2. K.O. validated
Matching control protein/peptide 399-0P
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).

Data sheet 399_003.pdf

References for Spinophilin - 399 003

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 399 003 DNA-PAINT; tested species: rat
Cat. No.: 399 003
Amount: 50 µg
Price: $375.00
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 399 003 DNA-PAINT; tested species: rat
Background
Spinophilin, also known as Neurabin II, is a scaffolding protein enriched in neuronal dendritic spines. It is involved in the targeted loacalization of protein phosphatase-1 (PP1), critical for dopaminergic modulation of glutamate neurotransmission.
More recently it has also been identified as a tumor suppressor. Loss of its function induces a proliferative response by increasing retinoblastoma protein (pRb) phosphorylation.