Spinophilin antibody - 399 003 K.O.

Spinophilin is enriched at dendritic spines

Rabbit polyclonal purified antibody

Cat. No.: 399 003

Amount: 50 µg

Price: $380.00

Cat. No. 399 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery DNA-PAINT: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Immunogen	Synthetic peptide corresponding to AA 290 to 302 from rat Spinophilin (UniProt Id: O35274)
Reactivity	Reacts with: rat (O35274), mouse (Q6R891). Other species not tested yet.
Specificity	Specific for Spinophilin isoform1. Does not detect isoform 2. K.O. validated
Matching control protein/peptide	399-0P
Remarks	DNA-PAINT: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Datasheet 399_003

References for Spinophilin - 399 003

Protocol for SUM-PAINT spatial proteomic imaging generating neuronal architecture maps in rat hippocampal neurons.
Unterauer EM, Schentarra EM, Jevdokimenko K, Boushehri SS, Marr C, Opazo F, Fornasiero EF, Jungmann R
STAR protocols (2025) 61: 103637. 399 003 DNA-PAINT; tested species: rat

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 399 003 DNA-PAINT; tested species: rat

Cat. No.: 399 003

Amount: 50 µg

Price: $380.00

ICC

IHC

IHC-P

Background

Spinophilin, also known as Neurabin II, is a scaffolding protein enriched in neuronal dendritic spines. It is involved in the targeted loacalization of protein phosphatase-1 (PP1), critical for dopaminergic modulation of glutamate neurotransmission.
More recently it has also been identified as a tumor suppressor. Loss of its function induces a proliferative response by increasing retinoblastoma protein (pRb) phosphorylation.

1 ) Brocos-Mosquera I et al., Prog. Neuropsychopharmacol. Biol. Psychiatry (2018)
2 ) Carnero A et al., Curr. Mol. Med. (2012)
3 ) Barnes AP et al., Brain Res. Mol. Brain Res. (2004)
4 ) Hsieh-Wilson LC et al., J. Biol. Chem. (2003)
5 ) Hsieh-Wilson LC et al., Biochemistry (1999)
6 ) Satoh A et al., J. Biol. Chem. (1998)
7 ) Allen PB et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

Protocols

ICC

Fixed Cells

IHC

IHC-P

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