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Home > Products > 272 011

Glycine transporter2 antibody - 272 011

Glycine transporters are transmembrane proteins involved in the removal of extracellular glycine
Mouse monoclonal purified IgG
Cat. No.: 272 011
Amount: 100 µg
Price: $415.00
Cat. No. 272 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: yes gallery  
IHC: 1 : 250 up to 1 : 500 gallery  
IHC-P: 1 : 100 up to 1 : 500 gallery  
Clone 117F12
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity Reacts with: mouse (Q761V0), rat (P58295).
Other species not tested yet.
Matching control protein/peptide 272-0P
Remarks

WB:  Glycine transporter 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 272_011.pdf
Cat. No.: 272 011
Amount: 100 µg
Price: $415.00
WB
ICC
IHC
IHC
IHC-P
Background
Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.
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