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Home > Products > 272 004

Glycine transporter2 antibody - 272 004 K.D.

Glycine transporters are transmembrane proteins involved in the removal of extracellular glycine
Guinea pig polyclonal antiserum
Cat. No.: 272 004
Amount: 100 µl
Price: $365.00
Cat. No. 272 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
EM: yes
Immunogen Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity Reacts with: rat (P58295), mouse (Q761V0).
Other species not tested yet.
Specificity K.D. validated PubMed: 30881475
Matching control protein/peptide 272-0P
Remarks

WB:  Glycine transporter 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 272_004.pdf

References for Glycine transporter2 - 272 004

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 272 004 ICC; tested species: rat
Multimodal sensory control of motor performance by glycinergic interneurons of the mouse spinal cord deep dorsal horn.
Gradwell MA, Ozeri-Engelhard N, Eisdorfer JT, Laflamme OD, Gonzalez M, Upadhyay A, Medlock L, Shrier T, Patel KR, Aoki A, Gandhi M, et al.
Neuron (2024) 1128: 1302-1327.e13. 272 004 IHC; tested species: mouse
Characterization of three cholinergic inputs to the cochlear nucleus.
Beebe NL, Herrera YN, Noftz WA, Roberts MT, Schofield BR
Journal of chemical neuroanatomy (2023) 131: 102284. 272 004 IHC; tested species: mouse
Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 272 004 IHC, EM; tested species: mouse
Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 272 004 IHC; tested species: rat
Group I metabotropic glutamate receptor-triggered temporally patterned action potential-dependent spontaneous synaptic transmission in mouse MNTB neurons.
Wang H, Peng K, Curry RJ, Li D, Wang Y, Wang X, Lu Y
Hearing research (2023) 435: 108822. 272 004 IHC; tested species: mouse
Neurotransmitter phenotype and axonal projection patterns of VIP-expressing neurons in the inferior colliculus.
Beebe NL, Silveira MA, Goyer D, Noftz WA, Roberts MT, Schofield BR
Journal of chemical neuroanatomy (2022) 126: 102189. 272 004 IHC; tested species: mouse
Morphological and neurochemical characterization of glycinergic neurons in laminae I-IV of the mouse spinal dorsal horn.
Miranda CO, Hegedüs K, Wildner H, Zeilhofer HU, Antal M
The Journal of comparative neurology (2021) : . 272 004 IHC; tested species: mouse
Cholinergic boutons are closely associated with excitatory cells and four subtypes of inhibitory cells in the inferior colliculus.
Beebe NL, Schofield BR
Journal of chemical neuroanatomy (2021) 116: 101998. 272 004 IHC; tested species: mouse
Opposing effects of an atypical glycinergic and substance P transmission on interpeduncular nucleus plasticity.
Melani R, Von Itter R, Jing D, Koppensteiner P, Ninan I
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2019) : . 272 004 IHC; tested species: mouse
Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse.
Tulloch AJ, Teo S, Carvajal BV, Tessier-Lavigne M, Jaworski A
The Journal of comparative neurology (2019) 52718: 2948-2972. 272 004 IHC; tested species: mouse
Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.
Bai F, Ma Y, Guo H, Li Y, Xu F, Zhang M, Dong H, Deng J, Xiong L
Evidence-based complementary and alternative medicine : eCAM (2019) 2019: 7493286. 272 004 IHC-P; KD verified; tested species: rat
Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 272 004 IHC, EM; tested species: mouse
Cat. No.: 272 004
Amount: 100 µl
Price: $365.00
Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 272 004 ICC; tested species: rat
Multimodal sensory control of motor performance by glycinergic interneurons of the mouse spinal cord deep dorsal horn.
Gradwell MA, Ozeri-Engelhard N, Eisdorfer JT, Laflamme OD, Gonzalez M, Upadhyay A, Medlock L, Shrier T, Patel KR, Aoki A, Gandhi M, et al.
Neuron (2024) 1128: 1302-1327.e13. 272 004 IHC; tested species: mouse
Characterization of three cholinergic inputs to the cochlear nucleus.
Beebe NL, Herrera YN, Noftz WA, Roberts MT, Schofield BR
Journal of chemical neuroanatomy (2023) 131: 102284. 272 004 IHC; tested species: mouse
Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 272 004 IHC, EM; tested species: mouse
Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 272 004 IHC; tested species: rat
Group I metabotropic glutamate receptor-triggered temporally patterned action potential-dependent spontaneous synaptic transmission in mouse MNTB neurons.
Wang H, Peng K, Curry RJ, Li D, Wang Y, Wang X, Lu Y
Hearing research (2023) 435: 108822. 272 004 IHC; tested species: mouse
Neurotransmitter phenotype and axonal projection patterns of VIP-expressing neurons in the inferior colliculus.
Beebe NL, Silveira MA, Goyer D, Noftz WA, Roberts MT, Schofield BR
Journal of chemical neuroanatomy (2022) 126: 102189. 272 004 IHC; tested species: mouse
Morphological and neurochemical characterization of glycinergic neurons in laminae I-IV of the mouse spinal dorsal horn.
Miranda CO, Hegedüs K, Wildner H, Zeilhofer HU, Antal M
The Journal of comparative neurology (2021) : . 272 004 IHC; tested species: mouse
Cholinergic boutons are closely associated with excitatory cells and four subtypes of inhibitory cells in the inferior colliculus.
Beebe NL, Schofield BR
Journal of chemical neuroanatomy (2021) 116: 101998. 272 004 IHC; tested species: mouse
Opposing effects of an atypical glycinergic and substance P transmission on interpeduncular nucleus plasticity.
Melani R, Von Itter R, Jing D, Koppensteiner P, Ninan I
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2019) : . 272 004 IHC; tested species: mouse
Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse.
Tulloch AJ, Teo S, Carvajal BV, Tessier-Lavigne M, Jaworski A
The Journal of comparative neurology (2019) 52718: 2948-2972. 272 004 IHC; tested species: mouse
Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.
Bai F, Ma Y, Guo H, Li Y, Xu F, Zhang M, Dong H, Deng J, Xiong L
Evidence-based complementary and alternative medicine : eCAM (2019) 2019: 7493286. 272 004 IHC-P; KD verified; tested species: rat
Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 272 004 IHC, EM; tested species: mouse
WB
ICC
IHC
IHC-P
Background
Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.
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