Chromogranin A antibody - 259 002

Chromogranin A is expressed at high levels in the neuroendocrine system

Rabbit polyclonal antiserum

Cat. No.: 259 002

Amount: 200 µl

Price: $355.00

Cat. No. 259 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 348 to 463 from mouse Chromogranin A (UniProt Id: P26339)
Reactivity	Reacts with: rat (P10354), mouse (P26339). Other species not tested yet.
Specificity	Specific chromogranin A.
Matching control protein/peptide	259-0P
Data sheet	259_002.pdf

Cat. No.: 259 002

Amount: 200 µl

Price: $355.00

Background

Chromogranin A (CgA) and B (CgB) are members of a family of acidic proteins stored and released throughout the neuroendocrine system. The large dense core vesicle associated proteins have multiple functions in neurons and neuroendocrine cells. They are differentially processed in different tissues.
Chromogranin A (CgA) is the precursor for the bioactive peptides pancreastatin, vasostatins, catestatin, β-granin and WE-14.

1 ) Domínguez N et al., J. Mol. Neurosci. (2012)
2 ) Park JJ et al., J. Cell. Sci. (2011)
3 ) Díaz-Vera J et al., J. Neurosci. (2010)
4 ) Schafer MK et al., Regul. Pept. (2010)
5 ) Machado JD et al., Cell. Mol. Neurobiol. (2010)
6 ) Montesinos MS et al., J. Neurosci. (2008)
7 ) Kim T et al., Cell (2001)
8 ) Natori S et al., EMBO J. (1998)
9 ) Kroesen S et al., Eur. J. Neurosci. (1996)
10 ) Wu HJ et al., J. Biol. Chem. (1991)

Protocols

OTHER

Preabsorbtion

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