Synaptic Systems - Chromogranin A

Cat. No.: 259 003

Amount: 50 µg

Price: $455.00

Cat. No. 259 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 348 to 463 from mouse Chromogranin A (UniProt Id: P26339)
Reactivity	Reacts with: rat (P10354), mouse (P26339), human (P10645). Other species not tested yet.
Specificity	K.O. validated PubMed: 29178418
Matching control protein/peptide	259-0P
Data sheet	259_003.pdf

References for Chromogranin A - 259 003

Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose.
Qian C, Wu Z, Sun R, Yu H, Zeng J, Rao Y, Li Y
eLife (2021) 10: . 259 003 WB, ICC; tested species: mouse

Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B.
Dominguez N, van Weering JRT, Borges R, Toonen RFG, Verhage M
Journal of neurochemistry (2018) 1443: 241-254. 259 003 WB; KO verified; tested species: mouse

Distinct Alterations in Dendritic Spine Morphology in the Absence of β-Neurexins.
Mohrmann L, Seebach J, Missler M, Rohlmann A
International journal of molecular sciences (2024) 252: . 259 003 ICC; tested species: mouse

High-throughput assay for regulated secretion of neuropeptides in mouse and human neurons.
Baginska U, Balagura G, Toonen RF, Verhage M
The Journal of biological chemistry (2024) : 107321. 259 003 ICC; tested species: human

Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 259 003 ICC; tested species: mouse

Prion protein conversion at two distinct cellular sites precedes fibrillisation.
Ribes JM, Patel MP, Halim HA, Berretta A, Tooze SA, Klöhn PC
Nature communications (2023) 141: 8354. 259 003 ICC; tested species: mouse

Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.
Nassal JP, Murphy FH, Toonen RF, Verhage M
eLife (2022) 11: . 259 003 ICC; tested species: mouse

Deletion of β-Neurexins in Mice Alters the Distribution of Dense-Core Vesicles in Presynapses of Hippocampal and Cerebellar Neurons.
Ferdos S, Brockhaus J, Missler M, Rohlmann A
Frontiers in neuroanatomy (2021) 15: 757017. 259 003 ICC; tested species: mouse

Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 259 003 ICC; tested species: rat

Pool size estimations for dense-core vesicles in mammalian CNS neurons.
Persoon CM, Moro A, Nassal JP, Farina M, Broeke JH, Arora S, Dominguez N, van Weering JR, Toonen RF, Verhage M
The EMBO journal (2018) : . 259 003 IHC; tested species: mouse

Cat. No.: 259 003

Amount: 50 µg

Price: $455.00

Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.
Nassal JP, Murphy FH, Toonen RF, Verhage M
eLife (2022) 11: . 259 003 ICC; tested species: mouse

ICC

IHC

IHC-P

Background

Chromogranin A (CgA) and B (CgB) are members of a family of acidic proteins stored and released throughout the neuroendocrine system. The large dense core vesicle associated proteins have multiple functions in neurons and neuroendocrine cells. They are differentially processed in different tissues.
Chromogranin A (CgA) is the precursor for the bioactive peptides pancreastatin, vasostatins, catestatin, β-granin and WE-14.

1 ) Domínguez N et al., J. Mol. Neurosci. (2012)
2 ) Park JJ et al., J. Cell. Sci. (2011)
3 ) Díaz-Vera J et al., J. Neurosci. (2010)
4 ) Schafer MK et al., Regul. Pept. (2010)
5 ) Machado JD et al., Cell. Mol. Neurobiol. (2010)
6 ) Montesinos MS et al., J. Neurosci. (2008)
7 ) Kim T et al., Cell (2001)
8 ) Natori S et al., EMBO J. (1998)
9 ) Kroesen S et al., Eur. J. Neurosci. (1996)
10 ) Wu HJ et al., J. Biol. Chem. (1991)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Chromogranin A antibody - 259 003 K.O.

References for Chromogranin A - 259 003