GluN2A/B antibody - 244 002

GluN2A and B are subunits of ionotropic NMDA type glutamate receptors

Rabbit polyclonal antiserum

Cat. No.: 244 002

Amount: 200 µl

Price: $355.00

Cat. No. 244 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not tested yet (see remarks) IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1448 to 1464 from mouse GluN2A (UniProt Id: P35436)
Reactivity	Reacts with: rat (Q00959, Q00960), mouse (P35436, Q01097). Other species not tested yet.
Specificity	Cross-reacts to GluN 2B due to sequence homology.
Matching control protein/peptide	244-0P
Remarks	IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. ICC: The affinity purified antibody (cat. no. 244 003) is recommended for this application.
Data sheet	244_002.pdf

Cat. No.: 244 002

Amount: 200 µl

Price: $355.00

Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca²⁺ influx and are thought to trigger Ca²⁺ dependent postsynaptic processes involved in long term potentiation and depression.

1 ) Groc L et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
2 ) Nagy GG et al., Eur. J. Neurosci. (2004)
3 ) Petralia RS et al., Eur. J. Neurosci. (2002)
4 ) Sans N et al., J. Neurosci. (2000)
5 ) Conti F et al., Cereb. Cortex (1999)
6 ) Petralia RS et al., J. Neurosci. (1994)
7 ) Monyer H et al., Science (1992)

Protocols

OTHER

Preabsorbtion

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