Synaptic Systems - GluN2A/B

Cat. No.: 244 003

Amount: 50 µg

Price: $455.00

Cat. No. 244 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 250 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1448 to 1464 from mouse GluN2A (UniProt Id: P35436)
Reactivity	Reacts with: rat (Q00959, Q00960), mouse (P35436, Q01097). Other species not tested yet.
Specificity	Cross-reacts to GluN 2B due to sequence homology.
Matching control protein/peptide	244-0P
Remarks	IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
Data sheet	244_003.pdf

References for GluN2A/B - 244 003

Astrocyte GluN2C NMDA receptors control basal synaptic strengths of hippocampal CA1 pyramidal neurons in the stratum radiatum.
Chipman PH, Fung CCA, Pazo Fernandez A, Sawant A, Tedoldi A, Kawai A, Ghimire Gautam S, Kurosawa M, Abe M, Sakimura K, Fukai T, et al.
eLife (2021) 10: . 244 003 WB; tested species: mouse

Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 244 003 WB, ICC; tested species: mouse

Cat. No.: 244 003

Amount: 50 µg

Price: $455.00

ICC

Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca²⁺ influx and are thought to trigger Ca²⁺ dependent postsynaptic processes involved in long term potentiation and depression.

1 ) Groc L et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
2 ) Nagy GG et al., Eur. J. Neurosci. (2004)
3 ) Petralia RS et al., Eur. J. Neurosci. (2002)
4 ) Sans N et al., J. Neurosci. (2000)
5 ) Conti F et al., Cereb. Cortex (1999)
6 ) Petralia RS et al., J. Neurosci. (1994)
7 ) Monyer H et al., Science (1992)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

GluN2A/B antibody - 244 003

References for GluN2A/B - 244 003