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GABA-A receptor alpha1 antibody extracellular - 224 218

GABA-A receptors are involved in inhibitory neurotransmission
Guinea pig monoclonal recombinant IgG
Cat. No.: 224 218
Amount: 50 µg
Price: $415.00
Cat. No. 224 218 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  
Clone Gp53D3
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Matching control protein/peptide 224-2P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody 53D3. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 224_218.pdf
Cat. No.: 224 218
Amount: 50 µg
Price: $415.00
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.