Home|||||||Technical Support|

GABA-A receptor alpha1 - 224 204

Receptors mediating inhibitory neurotransmission
Polyclonal Guinea pig antiserum
Cat. No.: 224 204
Amount: 100 µl
Price: 350.00 €
Cat. No. 224 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1.
Matching control protein/peptide 224-2P
Remarks

WB:  GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

Data sheet 224_204.pdf

References for GABA-A receptor alpha1 - 224 204

The primate-specific peptide Y-P30 regulates morphological maturation of neocortical dendritic spines.
Neumann JR, Dash-Wagh S, Jack A, Räk A, Jüngling K, Hamad MIK, Pape HC, Kreutz MR, Puskarjov M, Wahle P
PloS one (2019) 142: e0211151. 224 204 WB; tested species: rat
Cat. No.: 224 204
Quantity: 100 µl
Price: 350.00 €
The primate-specific peptide Y-P30 regulates morphological maturation of neocortical dendritic spines.
Neumann JR, Dash-Wagh S, Jack A, Räk A, Jüngling K, Hamad MIK, Pape HC, Kreutz MR, Puskarjov M, Wahle P
PloS one (2019) 142: e0211151. 224 204 WB; tested species: rat
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.