GABA-A receptor alpha1 antibody extracellular - 224 204

GABA-A receptors are involved in inhibitory neurotransmission

Guinea pig polyclonal antiserum

Cat. No.: 224 204

Amount: 100 µl

Price: $365.00

Cat. No. 224 204	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor α1. (UniProt Id: P62813)
Reactivity	Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.
Matching control protein/peptide	224-2P
Remarks	WB: GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	224_204.pdf

References for GABA-A receptor alpha1 - 224 204

The primate-specific peptide Y-P30 regulates morphological maturation of neocortical dendritic spines.
Neumann JR, Dash-Wagh S, Jack A, Räk A, Jüngling K, Hamad MIK, Pape HC, Kreutz MR, Puskarjov M, Wahle P
PloS one (2019) 142: e0211151. 224 204 WB; tested species: rat

Cat. No.: 224 204

Amount: 100 µl

Price: $365.00

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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