Synaptic Systems - GABA-A receptor alpha2

Cat. No.: 224 102

Amount: 200 µl

Price: $355.00

Cat. No. 224 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: yes (see remarks) IHC: yes (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity	Reacts with: human (P47869), rat (P23576), mouse (P26048). Other species not tested yet.
Matching control protein/peptide	224-1P
Remarks	WB: The crude antiserum is highly recommended. GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: The affinity purified antibody (cat.no. 224 103) is highly recommended for ICC. IHC: The affinity purified antibody (cat.no. 224 103) is highly recommended for this applications.
Data sheet	224_102.pdf

References for GABA-A receptor alpha2 - 224 102

Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 224 102 WB, ICC; tested species: rat

Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 102 WB

γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 102 WB

Aberrant expression of S-SCAM causes the loss of GABAergic synapses in hippocampal neurons.
Shin SM, Skaar S, Danielson E, Lee SH
Scientific reports (2020) 101: 83. 224 102 ICC; tested species: rat

Cat. No.: 224 102

Amount: 200 µl

Price: $355.00

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

OTHER

Preabsorbtion

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GABA-A receptor alpha2 antibody extracellular - 224 102

References for GABA-A receptor alpha2 - 224 102