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GABA-A receptor alpha2 - 224 102

Receptors mediating inhibitory neurotransmission
Polyclonal rabbit antiserum
Cat. No.: 224 102
Amount: 200 µl
Price: 350.00 €
Cat. No. 224 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: yes (see remarks)
IHC: yes (see remarks)
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity Reacts with: human (P47869), rat (P23576), mouse (P26048).
Other species not tested yet.
Specificity Specific for GABA-A receptor α2.
Matching control protein/peptide 224-1P
Remarks

WB: The crude antiserum is highly recommended. GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: The affinity purified antibody (cat.no. 224 103) is highly recommended for ICC.

IHC: The affinity purified antibody (cat.no. 224 103) is highly recommended for this applications.

Data sheet 224_102.pdf

References for GABA-A receptor alpha2 - 224 102

Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 224 102 WB, ICC; tested species: rat
Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 102 WB
γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 102 WB
Aberrant expression of S-SCAM causes the loss of GABAergic synapses in hippocampal neurons.
Shin SM, Skaar S, Danielson E, Lee SH
Scientific reports (2020) 101: 83. 224 102 ICC; tested species: rat
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 224 102 WB, ICC; tested species: rat
Cat. No.: 224 102
Quantity: 200 µl
Price: 350.00 €
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 224 102 WB, ICC; tested species: rat
Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 102 WB
γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 102 WB
Aberrant expression of S-SCAM causes the loss of GABAergic synapses in hippocampal neurons.
Shin SM, Skaar S, Danielson E, Lee SH
Scientific reports (2020) 101: 83. 224 102 ICC; tested species: rat
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 224 102 WB, ICC; tested species: rat
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.