|Cat. No. 224 102||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: yes (see remarks)
IHC: yes (see remarks)
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)|
Reacts with: human (P47869), rat (P23576), mouse (P26048).
Other species not tested yet.
|Specificity||Specific for GABA-A receptor α2.|
|Matching control protein/peptide||224-1P|
WB: The crude antiserum is highly recommended. GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: The affinity purified antibody (cat.no. 224 103) is highly recommended for ICC.
IHC: The affinity purified antibody (cat.no. 224 103) is highly recommended for this applications.
Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.