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Home > Products > 224 103

GABA-A receptor alpha2 antibody extracellular - 224 103 K.O.

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 103
Amount: 50 µg
Price: $405.00
Cat. No. 224 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes (see remarks)
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 gallery  
IHC-P: not tested yet
IHC-G: 1 : 500 (see remarks) gallery  
EM: yes
Immunogen Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity Reacts with: human (P47869), rat (P23576), mouse (P26048).
Other species not tested yet.
Specificity K.O. validated PubMed: 26973458
Matching control protein/peptide 224-1P
Remarks

WB: The crude antiserum (cat. no. 224 102) is highly recommended. GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.
IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017 is recommended.
Data sheet 224_103.pdf

References for GABA-A receptor alpha2 - 224 103

Sleep and wake cycles dynamically modulate hippocampal inhibitory synaptic plasticity.
Wu K, Han W, Lu W
PLoS biology (2022) 2011: e3001812. 224 103 WB; tested species: mouse
Inhibitory synapse dysfunction and epileptic susceptibility associated with KIF2A deletion in cortical interneurons.
Ruiz-Reig N, García-Sánchez D, Schakman O, Gailly P, Tissir F
Frontiers in molecular neuroscience (2022) 15: 1110986. 224 103 WB, IHC; tested species: mouse
REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion.
Prestigio C, Ferrante D, Marte A, Romei A, Lignani G, Onofri F, Valente P, Benfenati F, Baldelli P
eLife (2021) 10: . 224 103 WB; tested species: mouse
DNA repair enzyme NEIL3 enables a stable neural representation of space by shaping transcription in hippocampal neurons.
Kunath N, Bugaj AM, Bigonah P, Fernandez-Berrocal MS, Bjørås M, Ye J
iScience (2021) 2412: 103470. 224 103 WB, IHC-Fr; tested species: mouse
Network Neuromodulation of Opioid and GABAergic Receptors Following a Combination of "Juvenile" and "Adult Stress" in Rats.
Horovitz O, Ardi Z, Ashkenazi SK, Ritov G, Anunu R, Richter-Levin G
International journal of molecular sciences (2020) 2115: . 224 103 WB; tested species: rat
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 224 103 WB, ICC; tested species: mouse
Cat. No.: 224 103
Amount: 50 µg
Price: $405.00
ICC
IHC
IHC
IHC-G
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