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GABA-A receptor alpha2 - 224 103

Receptors mediating inhibitory neurotransmission
Polyclonal rabbit purified antibody
Cat. No.: 224 103
Amount: 50 µg
Price: 370.00 €
Cat. No. 224 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: yes (see remarks)
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes
Immunogen Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity Reacts with: human (P47869), rat (P23576), mouse (P26048).
Other species not tested yet.
Specificity Specific for GABA-A receptor α2. K.O. PubMed: 26973458
Matching control protein/peptide 224-1P
Remarks

WB: The crude antiserum (cat. no. 224 102) is highly recommended.

ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Data sheet 224_103.pdf

References for GABA-A receptor alpha2 - 224 103

Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 224 103 WB, ICC; tested species: mouse
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 224 103 WB, ICC; tested species: mouse
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 103 ICC
Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins.
Smith KR, Jones KA, Kopeikina KJ, Burette AC, Copits BA, Yoon S, Forrest MP, Fawcett-Patel JM, Hanley JG, Weinberg RJ, Swanson GT, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3746: 11127-11139. 224 103 ICC; tested species: rat
Gephyrin-binding peptides visualize postsynaptic sites and modulate neurotransmission.
Maric HM, Hausrat TJ, Neubert F, Dalby NO, Doose S, Sauer M, Kneussel M, Strømgaard K
Nature chemical biology (2017) 132: 153-160. 224 103 ICC; tested species: mouse
Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 224 103 ICC, IHC
Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory.
Hausrat TJ, Muhia M, Gerrow K, Thomas P, Hirdes W, Tsukita S, Heisler FF, Herich L, Dubroqua S, Breiden P, Feldon J, et al.
Nature communications (2015) 6: 6872. 224 103 ICC
GABAA receptor activity shapes the formation of inhibitory synapses between developing medium spiny neurons.
Arama J, Abitbol K, Goffin D, Fuchs C, Sihra TS, Thomson AM, Jovanovic JN
Frontiers in cellular neuroscience (2015) 9: 290. 224 103 ICC
Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.
Brady ML, Jacob TC
Developmental neurobiology (2015) 7511: 1241-51. 224 103 ICC; tested species: rat
Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments.
Linsalata AE, Chen X, Winters CA, Reese TS
The Journal of comparative neurology (2014) 5224: 921-36. 224 103 ICC; tested species: rat
Quantitative nanoscopy of inhibitory synapses: counting gephyrin molecules and receptor binding sites.
Specht CG, Izeddin I, Rodriguez PC, El Beheiry M, Rostaing P, Darzacq X, Dahan M, Triller A
Neuron (2013) 792: 308-21. 224 103 ICC; tested species: mouse
TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.
Pribiag H, Stellwagen D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3340: 15879-93. 224 103 ICC; tested species: rat
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 103 IHC; tested species: rat
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 224 103 IHC; tested species: mouse
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 103 IHC
αT-catenin in restricted brain cell types and its potential connection to autism.
Folmsbee SS, Wilcox DR, Tyberghein K, De Bleser P, Tourtellotte WG, van Hengel J, van Roy F, Gottardi CJ
Journal of molecular psychiatry (2016) 4: 2. 224 103 IHC
Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 224 103 ICC, IHC
Enrichment of GABAA Receptor α-Subunits on the Axonal Initial Segment Shows Regional Differences.
Gao Y, Heldt SA
Frontiers in cellular neuroscience (2016) 10: 39. 224 103 IHC; KO verified
GABAA receptor-mediated input change on orexin neurons following sleep deprivation in mice.
Matsuki T, Takasu M, Hirose Y, Murakoshi N, Sinton CM, Motoike T, Yanagisawa M
Neuroscience (2015) 284: 217-24. 224 103 IHC
Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala.
Tzanoulinou S, García-Mompó C, Castillo-Gómez E, Veenit V, Nacher J, Sandi C
PloS one (2014) 94: e94666. 224 103 IHC; tested species: rat
The effect of chronic administration of corticosterone on anxiety- and depression-like behavior and the expression of GABA-A receptor alpha-2 subunits in brain structures of low- and high-anxiety rats.
Skórzewska A, Lehner M, Wisłowska-Stanek A, Krząścik P, Ziemba A, Płaźnik A
Hormones and behavior (2014) 651: 6-13. 224 103 IHC; tested species: rat
Neural basis of benzodiazepine reward: requirement for α2 containing GABAA receptors in the nucleus accumbens.
Engin E, Bakhurin KI, Smith KS, Hines RM, Reynolds LM, Tang W, Sprengel R, Moss SJ, Rudolph U
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2014) 398: 1805-15. 224 103 IHC; KO verified; tested species: mouse
Changes in the brain expression of alpha-2 subunits of the GABA-A receptor after chronic restraint stress in low- and high-anxiety rats.
Wisłowska-Stanek A, Lehner M, Skórzewska A, Krząścik P, Maciejak P, Szyndler J, Ziemba A, Płaźnik A
Behavioural brain research (2013) 253: 337-45. 224 103 IHC; tested species: rat
Brain inflammation induces post-synaptic changes during early synapse formation in adult-born hippocampal neurons.
Chugh D, Nilsson P, Afjei SA, Bakochi A, Ekdahl CT
Experimental neurology (2013) 250: 176-88. 224 103 IHC; tested species: mouse
GABA(A) receptors containing the α2 subunit are critical for direction-selective inhibition in the retina.
Auferkorte ON, Baden T, Kaushalya SK, Zabouri N, Rudolph U, Haverkamp S, Euler T
PloS one (2012) 74: e35109. 224 103 IHC, EM; tested species: mouse,rabbit
Amacrine and bipolar inputs to midget and parasol ganglion cells in marmoset retina.
Abbott CJ, Percival KA, Martin PR, Grünert U
Visual neuroscience (2012) 293: 157-68. 224 103 IHC; tested species: marmoset
GABA(A) receptors containing the α2 subunit are critical for direction-selective inhibition in the retina.
Auferkorte ON, Baden T, Kaushalya SK, Zabouri N, Rudolph U, Haverkamp S, Euler T
PloS one (2012) 74: e35109. 224 103 IHC, EM; tested species: mouse,rabbit
Cat. No.: 224 103
Quantity: 50 µg
Price: 370.00 €
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 224 103 WB, ICC; tested species: mouse
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 224 103 WB, ICC; tested species: mouse
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 103 ICC
Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins.
Smith KR, Jones KA, Kopeikina KJ, Burette AC, Copits BA, Yoon S, Forrest MP, Fawcett-Patel JM, Hanley JG, Weinberg RJ, Swanson GT, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3746: 11127-11139. 224 103 ICC; tested species: rat
Gephyrin-binding peptides visualize postsynaptic sites and modulate neurotransmission.
Maric HM, Hausrat TJ, Neubert F, Dalby NO, Doose S, Sauer M, Kneussel M, Strømgaard K
Nature chemical biology (2017) 132: 153-160. 224 103 ICC; tested species: mouse
Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 224 103 ICC, IHC
Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory.
Hausrat TJ, Muhia M, Gerrow K, Thomas P, Hirdes W, Tsukita S, Heisler FF, Herich L, Dubroqua S, Breiden P, Feldon J, et al.
Nature communications (2015) 6: 6872. 224 103 ICC
GABAA receptor activity shapes the formation of inhibitory synapses between developing medium spiny neurons.
Arama J, Abitbol K, Goffin D, Fuchs C, Sihra TS, Thomson AM, Jovanovic JN
Frontiers in cellular neuroscience (2015) 9: 290. 224 103 ICC
Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.
Brady ML, Jacob TC
Developmental neurobiology (2015) 7511: 1241-51. 224 103 ICC; tested species: rat
Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments.
Linsalata AE, Chen X, Winters CA, Reese TS
The Journal of comparative neurology (2014) 5224: 921-36. 224 103 ICC; tested species: rat
Quantitative nanoscopy of inhibitory synapses: counting gephyrin molecules and receptor binding sites.
Specht CG, Izeddin I, Rodriguez PC, El Beheiry M, Rostaing P, Darzacq X, Dahan M, Triller A
Neuron (2013) 792: 308-21. 224 103 ICC; tested species: mouse
TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.
Pribiag H, Stellwagen D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3340: 15879-93. 224 103 ICC; tested species: rat
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 103 IHC; tested species: rat
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 224 103 IHC; tested species: mouse
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 103 IHC
αT-catenin in restricted brain cell types and its potential connection to autism.
Folmsbee SS, Wilcox DR, Tyberghein K, De Bleser P, Tourtellotte WG, van Hengel J, van Roy F, Gottardi CJ
Journal of molecular psychiatry (2016) 4: 2. 224 103 IHC
Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 224 103 ICC, IHC
Enrichment of GABAA Receptor α-Subunits on the Axonal Initial Segment Shows Regional Differences.
Gao Y, Heldt SA
Frontiers in cellular neuroscience (2016) 10: 39. 224 103 IHC; KO verified
GABAA receptor-mediated input change on orexin neurons following sleep deprivation in mice.
Matsuki T, Takasu M, Hirose Y, Murakoshi N, Sinton CM, Motoike T, Yanagisawa M
Neuroscience (2015) 284: 217-24. 224 103 IHC
Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala.
Tzanoulinou S, García-Mompó C, Castillo-Gómez E, Veenit V, Nacher J, Sandi C
PloS one (2014) 94: e94666. 224 103 IHC; tested species: rat
The effect of chronic administration of corticosterone on anxiety- and depression-like behavior and the expression of GABA-A receptor alpha-2 subunits in brain structures of low- and high-anxiety rats.
Skórzewska A, Lehner M, Wisłowska-Stanek A, Krząścik P, Ziemba A, Płaźnik A
Hormones and behavior (2014) 651: 6-13. 224 103 IHC; tested species: rat
Neural basis of benzodiazepine reward: requirement for α2 containing GABAA receptors in the nucleus accumbens.
Engin E, Bakhurin KI, Smith KS, Hines RM, Reynolds LM, Tang W, Sprengel R, Moss SJ, Rudolph U
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2014) 398: 1805-15. 224 103 IHC; KO verified; tested species: mouse
Changes in the brain expression of alpha-2 subunits of the GABA-A receptor after chronic restraint stress in low- and high-anxiety rats.
Wisłowska-Stanek A, Lehner M, Skórzewska A, Krząścik P, Maciejak P, Szyndler J, Ziemba A, Płaźnik A
Behavioural brain research (2013) 253: 337-45. 224 103 IHC; tested species: rat
Brain inflammation induces post-synaptic changes during early synapse formation in adult-born hippocampal neurons.
Chugh D, Nilsson P, Afjei SA, Bakochi A, Ekdahl CT
Experimental neurology (2013) 250: 176-88. 224 103 IHC; tested species: mouse
GABA(A) receptors containing the α2 subunit are critical for direction-selective inhibition in the retina.
Auferkorte ON, Baden T, Kaushalya SK, Zabouri N, Rudolph U, Haverkamp S, Euler T
PloS one (2012) 74: e35109. 224 103 IHC, EM; tested species: mouse,rabbit
Amacrine and bipolar inputs to midget and parasol ganglion cells in marmoset retina.
Abbott CJ, Percival KA, Martin PR, Grünert U
Visual neuroscience (2012) 293: 157-68. 224 103 IHC; tested species: marmoset
GABA(A) receptors containing the α2 subunit are critical for direction-selective inhibition in the retina.
Auferkorte ON, Baden T, Kaushalya SK, Zabouri N, Rudolph U, Haverkamp S, Euler T
PloS one (2012) 74: e35109. 224 103 IHC, EM; tested species: mouse,rabbit
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.