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Abeta 38/40/42/43 - 218 211BT

A major component of neuritic plaques in Alzheimer's disease
Monoclonal mouse purified IgG
Cat. No.: 218 211BT
Amount: 100 µg
Price: 465.00 €
Cat. No. 218 211BT 100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (see remarks)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 500 gallery  
ELISA: yes (see remarks)
Label biotin
Clone NT244
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 16 from human Abeta (UniProt Id: P05067)
Epitop Epitop: AA 4 to 8 from human Abeta (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 38, 40, 42, 43.
Remarks

WB: Boil membrane after blotting for 3min.

IHC: Antigen retrieval with formic acid is required.

ELISA: Suitable as detector antibody with cat.no. 218 203, 218 403 and 218 703 as capture antibodies.

Data sheet 218_211bt.pdf
Cat. No.: 218 211BT
Quantity: 100 µg
Price: 465.00 €
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta 38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.