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Abeta40 antibody - 218 203

Abeta peptides are major components of neuritic plaques in Alzheimer's disease
Rabbit polyclonal purified antibody
Cat. No.: 218 203
Amount: 50 µg
Price: $455.00
Cat. No. 218 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 500 (see remarks) gallery  
ELISA: yes (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.

Immunogen Synthetic peptide corresponding to AA 33 to 40 from human Abeta40 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 40, no cross reactivity to Abeta 38 and 42.
Remarks

WB: Detects purified Abeta 40. Complex samples like brain extracts still have to be tested. Boil membrane after blotting for 3min.
IHC: Antigen retrieval with formic acid is required.
IHC-P: Antigen retrieval with formic acid is required.
ELISA: suitable as capture and detector antibody for sandwich-ELISA.

Data sheet 218_203.pdf

References for Abeta40 - 218 203

Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal Aβ accumulation.
Christensen DZ, Huettenrauch M, Mitkovski M, Pradier L, Wirths O
Frontiers in aging neuroscience (2014) 6: 139. 218 203 IHC
Activation of the hypoxia response protects mice from amyloid-β accumulation.
Ollonen T, Kurkela M, Laitakari A, Sakko S, Koivisto H, Myllyharju J, Tanila H, Serpi R, Koivunen P
Cellular and molecular life sciences : CMLS (2022) 798: 432. 218 203 IHC-P; tested species: mouse
Cat. No.: 218 203
Amount: 50 µg
Price: $455.00
Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal Aβ accumulation.
Christensen DZ, Huettenrauch M, Mitkovski M, Pradier L, Wirths O
Frontiers in aging neuroscience (2014) 6: 139. 218 203 IHC
Activation of the hypoxia response protects mice from amyloid-β accumulation.
Ollonen T, Kurkela M, Laitakari A, Sakko S, Koivisto H, Myllyharju J, Tanila H, Serpi R, Koivunen P
Cellular and molecular life sciences : CMLS (2022) 798: 432. 218 203 IHC-P; tested species: mouse
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.