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GAD 1 / GAD 67 antibody - 198 003

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Polyclonal rabbit purified antibody
Cat. No.: 198 003
Amount: 50 µg
Price: $370.00
Cat. No. 198 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 3 to 101 from mouse GAD1 (UniProt Id: P48318)
Reactivity Reacts with: human (Q99259), rat (P18088), mouse (P48318).
Other species not tested yet.
Specificity Specific for GAD 1 / GAD 67.
Data sheet 198_003.pdf

References for GAD 1 / GAD 67 - 198 003

Distinct synaptic vesicle recycling in inhibitory nerve terminals is coordinated by SV2A.
Bae JR, Lee W, Jo YO, Han S, Koh S, Song WK, Kim SH
Progress in neurobiology (2020) : 101879. 198 003 ICC; tested species: rat
Accelerated intoxication of GABAergic synapses by botulinum neurotoxin A disinhibits stem cell-derived neuron networks prior to network silencing.
Beske PH, Scheeler SM, Adler M, McNutt PM
Frontiers in cellular neuroscience (2015) 9: 159. 198 003 ICC
Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons.
Horschitz S, Matthäus F, Groß A, Rosner J, Galach M, Greffrath W, Treede RD, Utikal J, Schloss P, Meyer-Lindenberg A
Stem cell research (2015) 151: 30-41. 198 003 ICC
Cat. No.: 198 003
Quantity: 50 µg
Price: $370.00
Distinct synaptic vesicle recycling in inhibitory nerve terminals is coordinated by SV2A.
Bae JR, Lee W, Jo YO, Han S, Koh S, Song WK, Kim SH
Progress in neurobiology (2020) : 101879. 198 003 ICC; tested species: rat
Accelerated intoxication of GABAergic synapses by botulinum neurotoxin A disinhibits stem cell-derived neuron networks prior to network silencing.
Beske PH, Scheeler SM, Adler M, McNutt PM
Frontiers in cellular neuroscience (2015) 9: 159. 198 003 ICC
Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons.
Horschitz S, Matthäus F, Groß A, Rosner J, Galach M, Greffrath W, Treede RD, Utikal J, Schloss P, Meyer-Lindenberg A
Stem cell research (2015) 151: 30-41. 198 003 ICC
Background

The glutamic acid decarboxylases GAD 1, also referred to as GAD 67, and GAD 2 / GAD 65 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.