Synaptic Systems

Cat. No.: 188 003

Amount: 50 µg

Price: $455.00

Cat. No. 188 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 250 up to 1 : 500 gallery IHC-P: 1 : 250 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)
Reactivity	Reacts with: human (P11137), rat (P15146), mouse (P20357). Other species not tested yet.
Specificity	Specific for MAP2; recognizes all four isoforms.
Matching control protein/peptide	188-0P
Remarks	WB: Due to its large size, MAP2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet	188_003.pdf

References for MAP2 - 188 003

Disease-associated polyalanine expansion mutations impair UBA6-dependent ubiquitination.
Amer-Sarsour F, Falik D, Berdichevsky Y, Kordonsky A, Eid S, Rabinski T, Ishtayeh H, Cohen-Adiv S, Braverman I, Blumen SC, Laviv T, et al.
The EMBO journal (2024) : . 188 003 ICC; tested species: mouse

Age-related deficits in neuronal physiology and cognitive function are recapitulated in young mice overexpressing the L-type calcium channel, CaV 1.3.
Moore SJ, Cazares VA, Temme SJ, Murphy GG
Aging cell (2023) 223: e13781. 188 003 ICC; tested species: mouse

Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.
Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, Scudieri P, et al.
International journal of molecular sciences (2022) 2318: . 188 003 ICC; tested species: human

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 188 003 ICC; tested species: human

TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 188 003 ICC; tested species: human

The C-terminal helix 9 motif in rat cannabinoid receptor type 1 regulates axonal trafficking and surface expression.
Fletcher-Jones A, Hildick KL, Evans AJ, Nakamura Y, Wilkinson KA, Henley JM
eLife (2019) 8: . 188 003 ICC; tested species: rat

Exosomes From Astrocyte Processes: Signaling to Neurons.
Venturini A, Passalacqua M, Pelassa S, Pastorino F, Tedesco M, Cortese K, Gagliani MC, Leo G, Maura G, Guidolin D, Agnati LF, et al.
Frontiers in pharmacology (2019) 10: 1452. 188 003 ICC; tested species: rat

Oxidative stress and altered mitochondrial protein expression in the absence of amyloid-β and tau pathology in iPSC-derived neurons from sporadic Alzheimer's disease patients.
Birnbaum JH, Wanner D, Gietl AF, Saake A, Kündig TM, Hock C, Nitsch RM, Tackenberg C
Stem cell research (2018) 27: 121-130. 188 003 ICC; tested species: human

An increase in basal BDNF provokes hyperactivation of the Akt-mammalian target of rapamycin pathway and deregulation of local dendritic translation in a mouse model of Down's syndrome.
Troca-Marín JA, Alves-Sampaio A, Montesinos ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3126: 9445-55. 188 003 ICC, IHC

GABAB receptor constituents revealed by tandem affinity purification from transgenic mice.
Bartoi T, Rigbolt KT, Du D, Köhr G, Blagoev B, Kornau HC
The Journal of biological chemistry (2010) 28527: 20625-33. 188 003 ICC

Microglia rescue neurons from aggregate-induced neuronal dysfunction and death through tunneling nanotubes.
Scheiblich H, Eikens F, Wischhof L, Opitz S, Jüngling K, Cserép C, Schmidt SV, Lambertz J, Bellande T, Pósfai B, Geck C, et al.
Neuron (2024) : . 188 003 IHC; tested species: mouse

An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 188 003 IHC; tested species: mouse

Cat. No.: 188 003

Amount: 50 µg

Price: $455.00

ICC

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP2 have been described so far.

1 ) Dehmelt L et al., Genome Biol. (2005)
2 ) Al-Bassam J et al., J. Cell Biol. (2002)
3 ) Di Stefano G et al., J. Histochem. Cytochem. (2001)
4 ) Chauhan N et al., Neurochem. Res. (1997)
5 ) Sánchez C et al., Biochem. J. (1995)
6 ) Izant JG et al., Proc. Natl. Acad. Sci. U.S.A. (1980)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

MAP2 antibody - 188 003

References for MAP2 - 188 003