Home|||||||Technical Support|

MAP 2 - 188 003

A major microtubule associated protein
Polyclonal rabbit purified antibody
Cat. No.: 188 003
Amount: 50 µg
Price: $450.00
Cat. No. 188 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 250 up to 1 : 500 gallery  
IHC-P/FFPE: 1 : 250 up to 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 2 to 314 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity Reacts with: human (P11137), rat (P15146), mouse (P20357).
Other species not tested yet.
Specificity Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide 188-0P
Remarks

WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 188_003.pdf

References for MAP 2 - 188 003

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 188 003 ICC; tested species: human
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 188 003 ICC; tested species: human
The C-terminal helix 9 motif in rat cannabinoid receptor type 1 regulates axonal trafficking and surface expression.
Fletcher-Jones A, Hildick KL, Evans AJ, Nakamura Y, Wilkinson KA, Henley JM
eLife (2019) 8: . 188 003 ICC; tested species: rat
Exosomes From Astrocyte Processes: Signaling to Neurons.
Venturini A, Passalacqua M, Pelassa S, Pastorino F, Tedesco M, Cortese K, Gagliani MC, Leo G, Maura G, Guidolin D, Agnati LF, et al.
Frontiers in pharmacology (2019) 10: 1452. 188 003 ICC; tested species: rat
Oxidative stress and altered mitochondrial protein expression in the absence of amyloid-β and tau pathology in iPSC-derived neurons from sporadic Alzheimer's disease patients.
Birnbaum JH, Wanner D, Gietl AF, Saake A, Kündig TM, Hock C, Nitsch RM, Tackenberg C
Stem cell research (2018) 27: 121-130. 188 003 ICC; tested species: human
An increase in basal BDNF provokes hyperactivation of the Akt-mammalian target of rapamycin pathway and deregulation of local dendritic translation in a mouse model of Down's syndrome.
Troca-Marín JA, Alves-Sampaio A, Montesinos ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3126: 9445-55. 188 003 ICC, IHC
GABAB receptor constituents revealed by tandem affinity purification from transgenic mice.
Bartoi T, Rigbolt KT, Du D, Köhr G, Blagoev B, Kornau HC
The Journal of biological chemistry (2010) 28527: 20625-33. 188 003 ICC
An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 188 003 IHC; tested species: mouse
An increase in basal BDNF provokes hyperactivation of the Akt-mammalian target of rapamycin pathway and deregulation of local dendritic translation in a mouse model of Down's syndrome.
Troca-Marín JA, Alves-Sampaio A, Montesinos ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3126: 9445-55. 188 003 ICC, IHC
Cat. No.: 188 003
Quantity: 50 µg
Price: $450.00
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 188 003 ICC; tested species: human
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 188 003 ICC; tested species: human
The C-terminal helix 9 motif in rat cannabinoid receptor type 1 regulates axonal trafficking and surface expression.
Fletcher-Jones A, Hildick KL, Evans AJ, Nakamura Y, Wilkinson KA, Henley JM
eLife (2019) 8: . 188 003 ICC; tested species: rat
Exosomes From Astrocyte Processes: Signaling to Neurons.
Venturini A, Passalacqua M, Pelassa S, Pastorino F, Tedesco M, Cortese K, Gagliani MC, Leo G, Maura G, Guidolin D, Agnati LF, et al.
Frontiers in pharmacology (2019) 10: 1452. 188 003 ICC; tested species: rat
Oxidative stress and altered mitochondrial protein expression in the absence of amyloid-β and tau pathology in iPSC-derived neurons from sporadic Alzheimer's disease patients.
Birnbaum JH, Wanner D, Gietl AF, Saake A, Kündig TM, Hock C, Nitsch RM, Tackenberg C
Stem cell research (2018) 27: 121-130. 188 003 ICC; tested species: human
An increase in basal BDNF provokes hyperactivation of the Akt-mammalian target of rapamycin pathway and deregulation of local dendritic translation in a mouse model of Down's syndrome.
Troca-Marín JA, Alves-Sampaio A, Montesinos ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3126: 9445-55. 188 003 ICC, IHC
GABAB receptor constituents revealed by tandem affinity purification from transgenic mice.
Bartoi T, Rigbolt KT, Du D, Köhr G, Blagoev B, Kornau HC
The Journal of biological chemistry (2010) 28527: 20625-33. 188 003 ICC
An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 188 003 IHC; tested species: mouse
An increase in basal BDNF provokes hyperactivation of the Akt-mammalian target of rapamycin pathway and deregulation of local dendritic translation in a mouse model of Down's syndrome.
Troca-Marín JA, Alves-Sampaio A, Montesinos ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3126: 9445-55. 188 003 ICC, IHC
Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP 2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP 2 have been described so far.