Synaptic Systems

Cat. No.: 188 002

Amount: 200 µl

Price: $365.00

Cat. No. 188 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: yes gallery IHC-P: 1 : 250 up to 1 : 500 gallery
Immunogen	Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)
Reactivity	Reacts with: human (P11137), rat (P15146), mouse (P20357), chicken. Other species not tested yet.
Specificity	Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide	188-0P
Remarks	WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet	188_002.pdf

References for MAP2 - 188 002

Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.
Hennegan J, Bryant AH, Griffiths L, Trigano M, Bartley OJM, Bartlett JJ, Minahan C, Abreu de Oliveira WA, Yutuc E, Ntikas S, Bartsocas CS, et al.
iScience (2024) 271: 108670. 188 002 ICC; tested species: human

Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 188 002 ICC; tested species: mouse

Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 188 002 ICC; tested species: mouse

Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response.
Manet C, Mansuroglu Z, Conquet L, Bortolin V, Comptdaer T, Segrt H, Bourdon M, Menidjel R, Stadler N, Tian G, Herit F, et al.
Journal of neuroinflammation (2022) 191: 307. 188 002 ICC; tested species: mouse

PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 188 002 ICC; tested species: mouse

IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Martins S, Müller-Schiffmann A, Erichsen L, Bohndorf M, Wruck W, Sleegers K, Van Broeckhoven C, Korth C, Adjaye J
International journal of molecular sciences (2020) 2112: . 188 002 ICC; tested species: human

Gabapentin inhibits multiple steps in the amyloid beta toxicity cascade.
González-Sanmiguel J, Burgos CF, Bascuñán D, Fernández-Pérez EJ, Riffo-Lepe N, Boopathi S, Fernández-Pérez A, Bobadilla-Azócar C, González W, Figueroa M, Vicente B, et al.
ACS chemical neuroscience (2020) : . 188 002 ICC; tested species: rat

Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 188 002 ICC; tested species: mouse

Matrix Metalloproteinase 14 Mediates APP Proteolysis and Lysosomal Alterations Induced by Oxidative Stress in Human Neuronal Cells.
Llorente P, Martins S, Sastre I, Aldudo J, Recuero M, Adjaye J, Bullido MJ
Oxidative medicine and cellular longevity (2020) 2020: 5917187. 188 002 ICC; tested species: human

Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 188 002 ICC; tested species: mouse

Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 188 002 ICC

The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 188 002 ICC

Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates.
Korotchenko S, Cingolani LA, Kuznetsova T, Bologna LL, Chiappalone M, Dityatev A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691654: 20140134. 188 002 ICC; tested species: mouse

Inefficient type I interferon-mediated antiviral protection of primary mouse neurons is associated with the lack of apolipoprotein l9 expression.
Kreit M, Paul S, Knoops L, De Cock A, Sorgeloos F, Michiels T
Journal of virology (2014) 887: 3874-84. 188 002 ICC; tested species: mouse

Somatodendritic serotonin release and re-uptake in mouse embryonic stem cell-derived serotonergic neurons.
Lau T, Schneidt T, Heimann F, Gundelfinger ED, Schloss P
Neurochemistry international (2010) 578: 969-78. 188 002 ICC