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MAP 2 - 188 002

A major microtubule associated protein
Polyclonal rabbit antiserum
Cat. No.: 188 002
Amount: 200 µl
Price: 350.00 €
Cat. No. 188 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: yes gallery  
IHC-P/FFPE: 1 : 250 up to 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 2 to 314 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity Reacts with: human (P11137), rat (P15146), mouse (P20357), chicken.
Other species not tested yet.
Specificity Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide 188-0P
Remarks

WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 188_002.pdf

References for MAP 2 - 188 002

Gabapentin inhibits multiple steps in the amyloid beta toxicity cascade.
González-Sanmiguel J, Burgos CF, Bascuñán D, Fernández-Pérez EJ, Riffo-Lepe N, Boopathi S, Fernández-Pérez A, Bobadilla-Azócar C, González W, Figueroa M, Vicente B, et al.
ACS chemical neuroscience (2020) : . 188 002 ICC; tested species: rat
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 188 002 ICC
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 188 002 ICC
Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates.
Korotchenko S, Cingolani LA, Kuznetsova T, Bologna LL, Chiappalone M, Dityatev A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691654: 20140134. 188 002 ICC; tested species: mouse
Inefficient type I interferon-mediated antiviral protection of primary mouse neurons is associated with the lack of apolipoprotein l9 expression.
Kreit M, Paul S, Knoops L, De Cock A, Sorgeloos F, Michiels T
Journal of virology (2014) 887: 3874-84. 188 002 ICC; tested species: mouse
Somatodendritic serotonin release and re-uptake in mouse embryonic stem cell-derived serotonergic neurons.
Lau T, Schneidt T, Heimann F, Gundelfinger ED, Schloss P
Neurochemistry international (2010) 578: 969-78. 188 002 ICC
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 002 IHC
Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 002 IHC
Neurological impairment in experimental antiphospholipid syndrome is associated with increased ligand binding to hippocampal and cortical serotonergic 5-HT1A receptors.
Frauenknecht K, Katzav A, Grimm C, Chapman J, Sommer CJ
Immunobiology (2013) 2184: 517-26. 188 002 IHC; tested species: mouse
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 188 002 IHC
The anaphase promoting complex is required for memory function in mice.
Kuczera T, Stilling RM, Hsia HE, Bahari-Javan S, Irniger S, Nasmyth K, Sananbenesi F, Fischer A
Learning & memory (Cold Spring Harbor, N.Y.) (2011) 181: 49-57. 188 002 IHC
Cat. No.: 188 002
Quantity: 200 µl
Price: 350.00 €
Gabapentin inhibits multiple steps in the amyloid beta toxicity cascade.
González-Sanmiguel J, Burgos CF, Bascuñán D, Fernández-Pérez EJ, Riffo-Lepe N, Boopathi S, Fernández-Pérez A, Bobadilla-Azócar C, González W, Figueroa M, Vicente B, et al.
ACS chemical neuroscience (2020) : . 188 002 ICC; tested species: rat
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 188 002 ICC
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 188 002 ICC
Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates.
Korotchenko S, Cingolani LA, Kuznetsova T, Bologna LL, Chiappalone M, Dityatev A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691654: 20140134. 188 002 ICC; tested species: mouse
Inefficient type I interferon-mediated antiviral protection of primary mouse neurons is associated with the lack of apolipoprotein l9 expression.
Kreit M, Paul S, Knoops L, De Cock A, Sorgeloos F, Michiels T
Journal of virology (2014) 887: 3874-84. 188 002 ICC; tested species: mouse
Somatodendritic serotonin release and re-uptake in mouse embryonic stem cell-derived serotonergic neurons.
Lau T, Schneidt T, Heimann F, Gundelfinger ED, Schloss P
Neurochemistry international (2010) 578: 969-78. 188 002 ICC
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 002 IHC
Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 002 IHC
Neurological impairment in experimental antiphospholipid syndrome is associated with increased ligand binding to hippocampal and cortical serotonergic 5-HT1A receptors.
Frauenknecht K, Katzav A, Grimm C, Chapman J, Sommer CJ
Immunobiology (2013) 2184: 517-26. 188 002 IHC; tested species: mouse
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 188 002 IHC
The anaphase promoting complex is required for memory function in mice.
Kuczera T, Stilling RM, Hsia HE, Bahari-Javan S, Irniger S, Nasmyth K, Sananbenesi F, Fischer A
Learning & memory (Cold Spring Harbor, N.Y.) (2011) 181: 49-57. 188 002 IHC
Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP 2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP 2 have been described so far.