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Home > Products > 187 003

P31 antibody - 187 003

P31 is a SNARE protein involved in retrograde Golgi to ER transport
Rabbit polyclonal purified antibody
Cat. No.: 187 003
Amount: 50 µg
Price: $375.00
Cat. No. 187 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 200 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 4 to 232 from human P31 (UniProt Id: Q9NZ43)
Reactivity Reacts with: human (Q9NZ43), rat, mouse (Q9CQ56).
Other species not tested yet.
Matching control protein/peptide 187-0P
Data sheet 187_003.pdf

References for P31 - 187 003

Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 187 003 WB; tested species: human
Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment.
Nieradka A, Ufer C, Thiadens K, Grech G, Horos R, van Coevorden-Hameete M, van den Akker E, Sofi S, Kuhn H, von Lindern M
PloS one (2014) 99: e104631. 187 003 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 187 003 WB
Cat. No.: 187 003
Amount: 50 µg
Price: $375.00
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 187 003 WB; tested species: human
Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment.
Nieradka A, Ufer C, Thiadens K, Grech G, Horos R, van Coevorden-Hameete M, van den Akker E, Sofi S, Kuhn H, von Lindern M
PloS one (2014) 99: e104631. 187 003 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 187 003 WB
WB
ICC
Background

Distinct sets of SNARE proteins mediate membrane-membrane fusion events between different intracellular compartments. P 31, a Use 1 homolog, is a Qc-SNARE protein and locates to the endoplasmatic reticulum (ER).Together with syntaxin 18, Sec 22b and BNIP 1 it mediates retrograde traffic from the Golgi to the ER.
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