Synaptic Systems - Neurofilament L

Cat. No.: 171 003BT

Amount: 100 µg

Price: $480.00

Simultaneous alterations of oligodendrocyte-specific CNP, astrocyte-specific AQP4 and neuronal NF-L demarcate ischemic tissue after experimental stroke in mice.
Mages B, Aleithe S, Blietz A, Krueger M, Härtig W, Michalski D
Neuroscience letters (2019) : 134405. 171 003BT IHC; tested species: mouse

WB

ICC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
4 ) Porchet R et al., Neuroreport (2003)
5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

WB

ICC

IHC

IHC-P

Other products for this target

Cat. No. 171 003BT	100 µg antibody, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 100 (see remarks) gallery IHC: 1 : 100 IHC-P: 1 : 100 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Immunogen	with AA 200-292 missing. Immunogen corresponds to AA 1 to 284 in AAH66952.1 (UniProt Id: P07196)
Reactivity	Reacts with: human (P07196), rat (P19527), mouse (P08551). Other species not tested yet.
Remarks	WB: This antibody is less sensitive than the crude rabbit antiserum (cat. no. 171 002) ICC: Methanol fixation is recommended.
Data sheet	171_003bt.pdf

Neurofilament L antibody - 171 003BT

References for Neurofilament L - 171 003BT