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Neurofilament L antibody - 171 003BT

Neurofilaments are major neuronal intermediate filaments
Polyclonal rabbit purified IgG
Cat. No.: 171 003BT
Amount: 100 µg
Price: $475.00
Cat. No. 171 003BT 100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 (see remarks) gallery  
IHC: 1 : 100
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery  
Label biotin
Immunogen Recombinant protein corresponding to AA 1 to 376 from human Neurofilament L (UniProt Id: P07196) with AA 200-292 missing. Immunogen corresponds to AA 1 to 284 in AAH66952.1
Reactivity Reacts with: human (P07196), rat (P19527), mouse (P08551).
Other species not tested yet.
Specificity Specific for neurofilament L.
Remarks

WB: This antibody is less sensitive than the crude rabbit antiserum (cat. no. 171 002)
ICC: Methanol fixation is recommended.

Data sheet 171_003bt.pdf

References for Neurofilament L - 171 003BT

Simultaneous alterations of oligodendrocyte-specific CNP, astrocyte-specific AQP4 and neuronal NF-L demarcate ischemic tissue after experimental stroke in mice.
Mages B, Aleithe S, Blietz A, Krueger M, Härtig W, Michalski D
Neuroscience letters (2019) : 134405. 171 003BT IHC; tested species: mouse
Cat. No.: 171 003BT
Quantity: 100 µg
Price: $475.00
Simultaneous alterations of oligodendrocyte-specific CNP, astrocyte-specific AQP4 and neuronal NF-L demarcate ischemic tissue after experimental stroke in mice.
Mages B, Aleithe S, Blietz A, Krueger M, Härtig W, Michalski D
Neuroscience letters (2019) : 134405. 171 003BT IHC; tested species: mouse
Background
Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.