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Home > Products > 171 002

Neurofilament L antibody - 171 002

Neurofilaments are major neuronal intermediate filaments
Rabbit polyclonal antiserum
Cat. No.: 171 002
Amount: 200 µl
Price: $360.00
Cat. No. 171 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 200 gallery  
iDISCO: 1 : 100

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info.
Immunogen with AA 200-292 missing. Immunogen corresponds to AA 1 to 284 in AAH66952.1 (UniProt Id: P07196)
Reactivity Reacts with: human (P07196), rat (P19527), mouse (P08551).
Other species not tested yet.
Data sheet 171_002.pdf

References for Neurofilament L - 171 002

Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose.
Chen XQ, Zuo X, Becker A, Head E, Mobley WC
Alzheimer's & dementia : the journal of the Alzheimer's Association (2022) : . 171 002 WB; tested species: human,mouse
Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 002 WB, IHC; tested species: human,mouse,rat
Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.
Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC
The Journal of experimental medicine (2016) 2134: 499-515. 171 002 WB, ICC; tested species: mouse
Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 171 002 ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 171 002 ICC; tested species: mouse
Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.
Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC
The Journal of experimental medicine (2016) 2134: 499-515. 171 002 WB, ICC; tested species: mouse
Differential antiapoptotic effect of erythropoietin on undifferentiated and retinoic acid-differentiated SH-SY5Y cells.
Wenker SD, Chamorro ME, Vota DM, Callero MA, Vittori DC, Nesse AB
Journal of cellular biochemistry (2010) 1101: 151-61. 171 002 ICC
Dipeptidyl peptidase 4 deficiency improves survival after focal cerebral ischemia in mice and ameliorates microglia activation and specific inflammatory markers.
Höfling C, Donkersloot P, Ulrich L, Burghardt S, Opitz M, Geissler S, Schilling S, Cynis H, Michalski D, Roßner S
Neurobiology of disease (2024) 201: 106671. 171 002 IHC; tested species: mouse
Catenin signaling controls phrenic motor neuron development and function during a narrow temporal window.
Vagnozzi AN, Moore MT, de Boer RL, Agarwal A, Zampieri N, Landmesser LT, Philippidou P
bioRxiv : the preprint server for biology (2023) : . 171 002 IHC; tested species: mouse
Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).
Härtig W, Meinicke A, Michalski D, Schob S, Jäger C
Frontiers in integrative neuroscience (2022) 16: 851988. 171 002 IHC; tested species: mouse
Microglial dyshomeostasis drives perineuronal net and synaptic loss in a CSF1R+/- mouse model of ALSP, which can be rescued via CSF1R inhibitors.
Arreola MA, Soni N, Crapser JD, Hohsfield LA, Elmore MRP, Matheos DP, Wood MA, Swarup V, Mortazavi A, Green KN
Science advances (2021) 735: . 171 002 IHC; tested species: mouse
Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia.
Oury J, Zhang W, Leloup N, Koide A, Corrado AD, Ketavarapu G, Hattori T, Koide S, Burden SJ
Nature (2021) : . 171 002 IHC; tested species: mouse
Classification of Microglial Morphological Phenotypes Using Machine Learning.
Leyh J, Paeschke S, Mages B, Michalski D, Nowicki M, Bechmann I, Winter K
Frontiers in cellular neuroscience (2021) 15: 701673. 171 002 IHC; tested species: mouse
Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor.
Straka T, Schröder C, Roos A, Kollipara L, Sickmann A, Williams MPI, Hafner M, Khan MM, Rudolf R
Frontiers in physiology (2021) 12: 626707. 171 002 IHC, iDISCO; tested species: mouse
Phrenic-specific transcriptional programs shape respiratory motor output.
Vagnozzi AN, Garg K, Dewitz C, Moore MT, Cregg JM, Jeannotte L, Zampieri N, Landmesser LT, Philippidou P
eLife (2020) 9: . 171 002 IHC; tested species: mouse
Neuropathological findings suggestive for a stroke in an alpaca (Vicugna pacos).
Schöniger S, Schütze E, Michalski D, Puchta J, Kaiser M, Härtig W
Acta veterinaria Scandinavica (2019) 611: 1. 171 002 IHC
MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses.
Oury J, Liu Y, Töpf A, Todorovic S, Hoedt E, Preethish-Kumar V, Neubert TA, Lin W, Lochmüller H, Burden SJ
The Journal of cell biology (2019) : . 171 002 IHC; tested species: mouse
Vezatin is Required for the Maturation of the Neuromuscular Synapse.
Koppel N, Friese MB, Cardasis HL, Neubert TA, Burden SJ
Molecular biology of the cell (2019) : mbcE19060313. 171 002 IHC; tested species: mouse
Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 002 WB, IHC; tested species: human,mouse,rat
Postnatal Development and Distribution of Sympathetic Innervation in Mouse Skeletal Muscle.
Straka T, Vita V, Prokshi K, Hörner SJ, Khan MM, Pirazzini M, Williams MPI, Hafner M, Zaglia T, Rudolf R
International journal of molecular sciences (2018) 197: . 171 002 IHC; tested species: mouse
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 171 002 IHC
Increasing MuSK activity delays denervation and improves motor function in ALS mice.
Pérez-García MJ, Burden SJ
Cell reports (2012) 23: 497-502. 171 002 IHC
Topoisomerase IIβ Selectively Regulates Motor Neuron Identity and Peripheral Connectivity through Hox/Pbx-Dependent Transcriptional Programs.
Edmond M, Hanley O, Philippidou P
eNeuro () 46: . 171 002 IHC; tested species: mouse
Gene Dosage Dependent Aggravation of the Neurological Phenotype in the 5XFAD Mouse Model of Alzheimer's Disease.
Richard BC, Kurdakova A, Baches S, Bayer TA, Weggen S, Wirths O
Journal of Alzheimer's disease : JAD (2015) 454: 1223-36. 171 002 IHC-P
Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor.
Straka T, Schröder C, Roos A, Kollipara L, Sickmann A, Williams MPI, Hafner M, Khan MM, Rudolf R
Frontiers in physiology (2021) 12: 626707. 171 002 IHC, iDISCO; tested species: mouse
Cat. No.: 171 002
Amount: 200 µl
Price: $360.00
Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose.
Chen XQ, Zuo X, Becker A, Head E, Mobley WC
Alzheimer's & dementia : the journal of the Alzheimer's Association (2022) : . 171 002 WB; tested species: human,mouse
Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 002 WB, IHC; tested species: human,mouse,rat
Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.
Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC
The Journal of experimental medicine (2016) 2134: 499-515. 171 002 WB, ICC; tested species: mouse
Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 171 002 ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 171 002 ICC; tested species: mouse
Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.
Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC
The Journal of experimental medicine (2016) 2134: 499-515. 171 002 WB, ICC; tested species: mouse
Differential antiapoptotic effect of erythropoietin on undifferentiated and retinoic acid-differentiated SH-SY5Y cells.
Wenker SD, Chamorro ME, Vota DM, Callero MA, Vittori DC, Nesse AB
Journal of cellular biochemistry (2010) 1101: 151-61. 171 002 ICC
Dipeptidyl peptidase 4 deficiency improves survival after focal cerebral ischemia in mice and ameliorates microglia activation and specific inflammatory markers.
Höfling C, Donkersloot P, Ulrich L, Burghardt S, Opitz M, Geissler S, Schilling S, Cynis H, Michalski D, Roßner S
Neurobiology of disease (2024) 201: 106671. 171 002 IHC; tested species: mouse
Catenin signaling controls phrenic motor neuron development and function during a narrow temporal window.
Vagnozzi AN, Moore MT, de Boer RL, Agarwal A, Zampieri N, Landmesser LT, Philippidou P
bioRxiv : the preprint server for biology (2023) : . 171 002 IHC; tested species: mouse
Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).
Härtig W, Meinicke A, Michalski D, Schob S, Jäger C
Frontiers in integrative neuroscience (2022) 16: 851988. 171 002 IHC; tested species: mouse
Microglial dyshomeostasis drives perineuronal net and synaptic loss in a CSF1R+/- mouse model of ALSP, which can be rescued via CSF1R inhibitors.
Arreola MA, Soni N, Crapser JD, Hohsfield LA, Elmore MRP, Matheos DP, Wood MA, Swarup V, Mortazavi A, Green KN
Science advances (2021) 735: . 171 002 IHC; tested species: mouse
Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia.
Oury J, Zhang W, Leloup N, Koide A, Corrado AD, Ketavarapu G, Hattori T, Koide S, Burden SJ
Nature (2021) : . 171 002 IHC; tested species: mouse
Classification of Microglial Morphological Phenotypes Using Machine Learning.
Leyh J, Paeschke S, Mages B, Michalski D, Nowicki M, Bechmann I, Winter K
Frontiers in cellular neuroscience (2021) 15: 701673. 171 002 IHC; tested species: mouse
Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor.
Straka T, Schröder C, Roos A, Kollipara L, Sickmann A, Williams MPI, Hafner M, Khan MM, Rudolf R
Frontiers in physiology (2021) 12: 626707. 171 002 IHC, iDISCO; tested species: mouse
Phrenic-specific transcriptional programs shape respiratory motor output.
Vagnozzi AN, Garg K, Dewitz C, Moore MT, Cregg JM, Jeannotte L, Zampieri N, Landmesser LT, Philippidou P
eLife (2020) 9: . 171 002 IHC; tested species: mouse
Neuropathological findings suggestive for a stroke in an alpaca (Vicugna pacos).
Schöniger S, Schütze E, Michalski D, Puchta J, Kaiser M, Härtig W
Acta veterinaria Scandinavica (2019) 611: 1. 171 002 IHC
MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses.
Oury J, Liu Y, Töpf A, Todorovic S, Hoedt E, Preethish-Kumar V, Neubert TA, Lin W, Lochmüller H, Burden SJ
The Journal of cell biology (2019) : . 171 002 IHC; tested species: mouse
Vezatin is Required for the Maturation of the Neuromuscular Synapse.
Koppel N, Friese MB, Cardasis HL, Neubert TA, Burden SJ
Molecular biology of the cell (2019) : mbcE19060313. 171 002 IHC; tested species: mouse
Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 002 WB, IHC; tested species: human,mouse,rat
Postnatal Development and Distribution of Sympathetic Innervation in Mouse Skeletal Muscle.
Straka T, Vita V, Prokshi K, Hörner SJ, Khan MM, Pirazzini M, Williams MPI, Hafner M, Zaglia T, Rudolf R
International journal of molecular sciences (2018) 197: . 171 002 IHC; tested species: mouse
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 171 002 IHC
Increasing MuSK activity delays denervation and improves motor function in ALS mice.
Pérez-García MJ, Burden SJ
Cell reports (2012) 23: 497-502. 171 002 IHC
Topoisomerase IIβ Selectively Regulates Motor Neuron Identity and Peripheral Connectivity through Hox/Pbx-Dependent Transcriptional Programs.
Edmond M, Hanley O, Philippidou P
eNeuro () 46: . 171 002 IHC; tested species: mouse
Gene Dosage Dependent Aggravation of the Neurological Phenotype in the 5XFAD Mouse Model of Alzheimer's Disease.
Richard BC, Kurdakova A, Baches S, Bayer TA, Weggen S, Wirths O
Journal of Alzheimer's disease : JAD (2015) 454: 1223-36. 171 002 IHC-P
Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor.
Straka T, Schröder C, Roos A, Kollipara L, Sickmann A, Williams MPI, Hafner M, Khan MM, Rudolf R
Frontiers in physiology (2021) 12: 626707. 171 002 IHC, iDISCO; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC-P
Background
Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.
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