Synaptic Systems

Cat. No.: 162 002

Amount: 200 µl

Price: $355.00

Cat. No. 162 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 135 to 149 from rat Shank1 (UniProt Id: Q9WV48)
Reactivity	Reacts with: human (Q9Y566), rat (Q9WV48), mouse (D3YZU1), dog, pig, cow, monkey. Other species not tested yet.
Data sheet	162_002.pdf

References for Shank1 - 162 002

Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 162 002 WB; tested species: mouse

Adult restoration of Shank3 expression rescues selective autistic-like phenotypes.
Mei Y, Monteiro P, Zhou Y, Kim JA, Gao X, Fu Z, Feng G
Nature (2016) 5307591: 481-4. 162 002 WB; tested species: mouse

Hyperactivity of newborn Pten knock-out neurons results from increased excitatory synaptic drive.
Williams MR, DeSpenza T, Li M, Gulledge AT, Luikart BW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 353: 943-59. 162 002 IHC

Cat. No.: 162 002

Amount: 200 µl

Price: $355.00

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

Other products for this target

Shank1 antibody - 162 002

References for Shank1 - 162 002