Synaptic Systems - Synaptojanin1

Cat. No.: 145 103

Amount: 50 µg

Price: $375.00

Cat. No. 145 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 200 up to 1 : 2000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 5000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1140 to 1155 from rat Synaptojanin1 (UniProt Id: Q62910-1)
Reactivity	Reacts with: rat (Q62910-1, Q62910-2, Q62910-3), mouse (Q8CHC4), hamster. Other species not tested yet.
Specificity	Recognizes isoforms 1, 2, 3 of synaptojanin 1.
Matching control protein/peptide	145-1P
Remarks	WB: Running SDS-PAGE with non-boiled samples gives stronger signals.
Data sheet	145_103.pdf

References for Synaptojanin1 - 145 103

Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 145 103 WB; tested species: mouse

Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 145 103 IHC; tested species: mouse

Cat. No.: 145 103

Amount: 50 µg

Price: $375.00

IHC

Background

Synaptojanin 1 is a phosphoinositide phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). The 145 kDa isoform is predominantly expressed in the nervous system. It has a three domain structure with an N-terminal part homologous to the yeast Sac 1p protein, a central inositol 5-phosphatase domain and a C-terminal SH3 binding domain. Synaptojanin is involved in clathrin mediated synaptic vesicle recycling and binds to endophilin and amphiphysin.
Six isoforms are described so far and two splice variants of the 145 kDa isoform. The predominant one contains a 16 AA insert at position 1140-1155.

1 ) Lee SY et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
2 ) Schuske KR et al., Neuron (2003)
3 ) Verstreken P et al., Neuron (2003)
4 ) Song W et al., Neuron (2003)
5 ) Luthi A et al., J. Neurosci. (2001)
6 ) Harris TW et al., J. Cell Biol. (2000)
7 ) Haffner C et al., Curr. Biol. (2000)
8 ) Woscholski R et al., J. Biol. Chem. (1997)
9 ) de Heuvel E et al., J. Biol. Chem. (1997)
10 ) Ramjaun AR et al., J. Biol. Chem. (1996)

Protocols

IHC

OTHER

Preabsorbtion

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Synaptojanin1 antibody splice variant - 145 103

References for Synaptojanin1 - 145 103