Synaptic Systems - Synaptojanin1

Cat. No.: 145-1P

Amount: 100 µg

Price: $105.00

Background

Synaptojanin 1 is a phosphoinositide phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). The 145 kDa isoform is predominantly expressed in the nervous system. It has a three domain structure with an N-terminal part homologous to the yeast Sac 1p protein, a central inositol 5-phosphatase domain and a C-terminal SH3 binding domain. Synaptojanin is involved in clathrin mediated synaptic vesicle recycling and binds to endophilin and amphiphysin.
Six isoforms are described so far and two splice variants of the 145 kDa isoform. The predominant one contains a 16 AA insert at position 1140-1155.

1 ) Lee SY et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
2 ) Schuske KR et al., Neuron (2003)
3 ) Verstreken P et al., Neuron (2003)
4 ) Song W et al., Neuron (2003)
5 ) Luthi A et al., J. Neurosci. (2001)
6 ) Harris TW et al., J. Cell Biol. (2000)
7 ) Haffner C et al., Curr. Biol. (2000)
8 ) Woscholski R et al., J. Biol. Chem. (1997)
9 ) de Heuvel E et al., J. Biol. Chem. (1997)
10 ) Ramjaun AR et al., J. Biol. Chem. (1996)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 145-1P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 1140 to 1155 from rat Synaptojanin1 (UniProt Id: Q62910-1)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	145 103
Remarks	This control peptide consists of the synthetic peptide (CGVGAPPSPGVTRREME) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	145-1p.pdf

Synaptojanin1 peptide - 145-1P