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Synaptojanin1 peptide - 145-1P

Synaptojanin1 is a phosphoinositide phosphatase
control peptide
Cat. No.: 145-1P
Amount: 100 µg
Price: $105.00
Cat. No. 145-1P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
Immunogen Synthetic peptide corresponding to AA 1140 to 1155 from rat Synaptojanin1 (UniProt Id: Q62910-1)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 145 103
Remarks

This control peptide consists of the synthetic peptide (CGVGAPPSPGVTRREME) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 145-1p.pdf
Cat. No.: 145-1P
Amount: 100 µg
Price: $105.00
Background

Synaptojanin 1 is a phosphoinositide phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). The 145 kDa isoform is predominantly expressed in the nervous system. It has a three domain structure with an N-terminal part homologous to the yeast Sac 1p protein, a central inositol 5-phosphatase domain and a C-terminal SH3 binding domain. Synaptojanin is involved in clathrin mediated synaptic vesicle recycling and binds to endophilin and amphiphysin.
Six isoforms are described so far and two splice variants of the 145 kDa isoform. The predominant one contains a 16 AA insert at position 1140-1155.

Protocols