Synaptic Systems

Cat. No.: 135 411

Amount: 100 µg

Price: $415.00

Cat. No. 135 411	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: not recommended IHC: not recommended IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	321A8
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Epitop	AA 564 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity	Reacts with: rat (Q9JI12). No signal: chicken. Other species not tested yet.
Remarks	WB: Cat. no. 135 421 is more sensitive and the first choice for westernblot experiments. VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 135 403 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	135_411.pdf

References for VGLUT2 - 135 411

Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 135 411 WB; tested species: mouse

Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 135 411 IP

A sandwich enzyme-linked immunosorbent assay for the quantification of insoluble membrane and scaffold proteins.
Geumann C, Grønborg M, Hellwig M, Martens H, Jahn R
Analytical biochemistry (2010) 4022: 161-9. 135 411 IP

Carnosic acid improves outcome after repetitive mild traumatic brain injury.
Maynard ME, Underwood EL, Redell JB, Zhao J, Kobori N, Hood KN, Moore AN, Dash PK
Journal of neurotrauma (2019) : . 135 411 IHC; tested species: mouse

Synaptic regulation by OPRM1 variants in reward neurocircuitry.
Popova D, Desai N, Blendy JA, Pang ZP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 135 411 IHC; tested species: mouse

Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE ε4.
Postupna N, Latimer CS, Larson EB, Sherfield E, Paladin J, Shively CA, Jorgensen MJ, Andrews RN, Kaplan JR, Crane PK, Montine KS, et al.
The American journal of pathology (2017) 1874: 884-895. 135 411 FACS; tested species: human

Cat. No.: 135 411

Amount: 100 µg

Price: $415.00

Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

1 ) Takamori S et al., J. Neurosci. (2001)
2 ) Fremeau RT et al., Neuron (2001)
3 ) Fujiyama F et al., J. Comp. Neurol. (2001)
4 ) Aihara Y et al., J. Neurochem. (2000)

Protocols

Standard protocol

ELISA

Membrane proteins

Other products for this target

VGLUT2 antibody - 135 411

References for VGLUT2 - 135 411