Cat. No.: 135 411
Amount: 100 µg
Price:
$415.00
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| Cat. No. 135 411 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: yes ICC: not recommended IHC: not recommended IHC-P: not tested yet ELISA: yes (see remarks) |
| Clone | 321A8 |
| Subtype | IgG2a (κ light chain) |
| Immunogen | Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12) |
| Epitop |
Epitop: AA 564 to 582 from rat VGLUT2 (UniProt Id: Q9JI12) |
| Reactivity |
Reacts with: rat (Q9JI12). No signal: chicken. Other species not tested yet. |
| Remarks |
WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
| Data sheet | 135_411.pdf |
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The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.