Tailor-made Antibodies
and Tools for Life Science

VGLUT2 antibody - 135 411

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 135 411
Amount: 100 µg
Price: $420.00
Cat. No. 135 411 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet
ELISA: yes (see remarks)
Clone 321A8
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Epitop AA 564 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12).
No signal: chicken.
Other species not tested yet.
Remarks

WB

  • Cat. no. 135 421 is more sensitive and the first choice for westernblot experiments.
  • VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 135 403 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 135_411.pdf

References for VGLUT2 - 135 411

Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 135 411 WB; tested species: mouse
Cat. No.: 135 411
Amount: 100 µg
Price: $420.00