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VGLUT 2 - 135 411

Glutamate transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 135 411
Amount: 100 µg
Price: $415.00
Cat. No. 135 411 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
Clone 321A8
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 510 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Epitop Epitop: AA 564 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12).
No signal: chicken.
Other species not tested yet.
Specificity Specific for VGLUT 2.
Matching control protein/peptide 135-4P
Remarks

WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 135 403 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 135_411.pdf

References for VGLUT 2 - 135 411

Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 135 411 WB; tested species: mouse
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 135 411 IP
A sandwich enzyme-linked immunosorbent assay for the quantification of insoluble membrane and scaffold proteins.
Geumann C, Grønborg M, Hellwig M, Martens H, Jahn R
Analytical biochemistry (2010) 4022: 161-9. 135 411 IP
Carnosic acid improves outcome after repetitive mild traumatic brain injury.
Maynard ME, Underwood EL, Redell JB, Zhao J, Kobori N, Hood KN, Moore AN, Dash PK
Journal of neurotrauma (2019) : . 135 411 IHC; tested species: mouse
Synaptic regulation by OPRM1 variants in reward neurocircuitry.
Popova D, Desai N, Blendy JA, Pang ZP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 135 411 IHC; tested species: mouse
Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE ε4.
Postupna N, Latimer CS, Larson EB, Sherfield E, Paladin J, Shively CA, Jorgensen MJ, Andrews RN, Kaplan JR, Crane PK, Montine KS, et al.
The American journal of pathology (2017) 1874: 884-895. 135 411 FACS; tested species: human
Cat. No.: 135 411
Quantity: 100 µg
Price: $415.00
Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 135 411 WB; tested species: mouse
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 135 411 IP
A sandwich enzyme-linked immunosorbent assay for the quantification of insoluble membrane and scaffold proteins.
Geumann C, Grønborg M, Hellwig M, Martens H, Jahn R
Analytical biochemistry (2010) 4022: 161-9. 135 411 IP
Carnosic acid improves outcome after repetitive mild traumatic brain injury.
Maynard ME, Underwood EL, Redell JB, Zhao J, Kobori N, Hood KN, Moore AN, Dash PK
Journal of neurotrauma (2019) : . 135 411 IHC; tested species: mouse
Synaptic regulation by OPRM1 variants in reward neurocircuitry.
Popova D, Desai N, Blendy JA, Pang ZP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 135 411 IHC; tested species: mouse
Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE ε4.
Postupna N, Latimer CS, Larson EB, Sherfield E, Paladin J, Shively CA, Jorgensen MJ, Andrews RN, Kaplan JR, Crane PK, Montine KS, et al.
The American journal of pathology (2017) 1874: 884-895. 135 411 FACS; tested species: human
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.