Home|||||||Technical Support|

VGLUT 2 - 135 421

Glutamate transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 135 421
Amount: 100 µg
Price: $415.00
Cat. No. 135 421 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Clone 95E11
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Epitop Epitop: AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
Specificity Specific for VGLUT 2.
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_421.pdf

References for VGLUT 2 - 135 421

Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 135 421 WB; tested species: mouse
TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy.
Atkinson R, Leung J, Bender J, Kirkcaldie M, Vickers J, King A
Disease models & mechanisms (2021) : . 135 421 IHC; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 135 421 IHC; tested species: rat
Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1.
Wang L, Kloc M, Maher E, Erisir A, Maffei A
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 135 421 IHC; tested species: rat
Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivo.
Dhar M, Hantman AW, Nishiyama H
Scientific reports (2018) 81: 17561. 135 421 IHC; tested species: mouse
Assembly of Excitatory Synapses in the Absence of Glutamatergic Neurotransmission.
Sando R, Bushong E, Zhu Y, Huang M, Considine C, Phan S, Ju S, Uytiepo M, Ellisman M, Maximov A
Neuron (2017) 942: 312-321.e3. 135 421 IHC; tested species: mouse
Cat. No.: 135 421
Quantity: 100 µg
Price: $415.00
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 135 421 WB; tested species: mouse
TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy.
Atkinson R, Leung J, Bender J, Kirkcaldie M, Vickers J, King A
Disease models & mechanisms (2021) : . 135 421 IHC; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 135 421 IHC; tested species: rat
Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1.
Wang L, Kloc M, Maher E, Erisir A, Maffei A
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 135 421 IHC; tested species: rat
Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivo.
Dhar M, Hantman AW, Nishiyama H
Scientific reports (2018) 81: 17561. 135 421 IHC; tested species: mouse
Assembly of Excitatory Synapses in the Absence of Glutamatergic Neurotransmission.
Sando R, Bushong E, Zhu Y, Huang M, Considine C, Phan S, Ju S, Uytiepo M, Ellisman M, Maximov A
Neuron (2017) 942: 312-321.e3. 135 421 IHC; tested species: mouse
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.