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Home > Products > 135 421

VGLUT2 antibody - 135 421

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 135 421
Amount: 100 µg
Price: $420.00
Cat. No. 135 421 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
IHC-G: (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone 95E11
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
Matching control protein/peptide 135-4P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
Data sheet 135_421.pdf

References for VGLUT2 - 135 421

Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.
Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, Scudieri P, et al.
International journal of molecular sciences (2022) 2318: . 135 421 WB; tested species: human
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 421 WB, UPTAKE; tested species: rat
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 135 421 WB; tested species: mouse
Excitatory synaptic structural abnormalities produced by templated aggregation of α-syn in the basolateral amygdala.
Gcwensa NZ, Russell DL, Long KY, Brzozowski CF, Liu X, Gamble KL, Cowell RM, Volpicelli-Daley LA
Neurobiology of disease (2024) 199: 106595. 135 421 IHC; tested species: mouse
Tonotopic differentiation of presynaptic neurotransmitter-releasing machinery in the auditory brainstem during the prehearing period and its selective deficits in Fmr1 knockout mice.
Yu X, Wang Y
The Journal of comparative neurology (2022) 53018: 3248-3269. 135 421 IHC; tested species: mouse
Sarm1 knockout modifies biomarkers of neurodegeneration and spinal cord circuitry but not disease progression in the mSOD1G93A mouse model of ALS.
Collins JM, Atkinson RAK, Matthews LM, Murray IC, Perry SE, King AE
Neurobiology of disease (2022) 172: 105821. 135 421 IHC; tested species: mouse
TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy.
Atkinson R, Leung J, Bender J, Kirkcaldie M, Vickers J, King A
Disease models & mechanisms (2021) : . 135 421 IHC; tested species: mouse
In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.
Tai W, Wu W, Wang LL, Ni H, Chen C, Yang J, Zang T, Zou Y, Xu XM, Zhang CL
Cell stem cell (2021) 285: 923-937.e4. 135 421 IHC; tested species: mouse
Adult medial habenula neurons require GDNF receptor GFRα1 for synaptic stability and function.
Fernández-Suárez D, Krapacher FA, Pietrajtis K, Andersson A, Kisiswa L, Carrier-Ruiz A, Diana MA, Ibáñez CF
PLoS biology (2021) 1911: e3001350. 135 421 IHC; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 135 421 IHC; tested species: rat
Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1.
Wang L, Kloc M, Maher E, Erisir A, Maffei A
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 135 421 IHC; tested species: rat
Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivo.
Dhar M, Hantman AW, Nishiyama H
Scientific reports (2018) 81: 17561. 135 421 IHC; tested species: mouse
Assembly of Excitatory Synapses in the Absence of Glutamatergic Neurotransmission.
Sando R, Bushong E, Zhu Y, Huang M, Considine C, Phan S, Ju S, Uytiepo M, Ellisman M, Maximov A
Neuron (2017) 942: 312-321.e3. 135 421 IHC; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 421 WB, UPTAKE; tested species: rat
Cat. No.: 135 421
Amount: 100 µg
Price: $420.00
Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.
Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, Scudieri P, et al.
International journal of molecular sciences (2022) 2318: . 135 421 WB; tested species: human
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 421 WB, UPTAKE; tested species: rat
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 135 421 WB; tested species: mouse
Excitatory synaptic structural abnormalities produced by templated aggregation of α-syn in the basolateral amygdala.
Gcwensa NZ, Russell DL, Long KY, Brzozowski CF, Liu X, Gamble KL, Cowell RM, Volpicelli-Daley LA
Neurobiology of disease (2024) 199: 106595. 135 421 IHC; tested species: mouse
Tonotopic differentiation of presynaptic neurotransmitter-releasing machinery in the auditory brainstem during the prehearing period and its selective deficits in Fmr1 knockout mice.
Yu X, Wang Y
The Journal of comparative neurology (2022) 53018: 3248-3269. 135 421 IHC; tested species: mouse
Sarm1 knockout modifies biomarkers of neurodegeneration and spinal cord circuitry but not disease progression in the mSOD1G93A mouse model of ALS.
Collins JM, Atkinson RAK, Matthews LM, Murray IC, Perry SE, King AE
Neurobiology of disease (2022) 172: 105821. 135 421 IHC; tested species: mouse
TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy.
Atkinson R, Leung J, Bender J, Kirkcaldie M, Vickers J, King A
Disease models & mechanisms (2021) : . 135 421 IHC; tested species: mouse
In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.
Tai W, Wu W, Wang LL, Ni H, Chen C, Yang J, Zang T, Zou Y, Xu XM, Zhang CL
Cell stem cell (2021) 285: 923-937.e4. 135 421 IHC; tested species: mouse
Adult medial habenula neurons require GDNF receptor GFRα1 for synaptic stability and function.
Fernández-Suárez D, Krapacher FA, Pietrajtis K, Andersson A, Kisiswa L, Carrier-Ruiz A, Diana MA, Ibáñez CF
PLoS biology (2021) 1911: e3001350. 135 421 IHC; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 135 421 IHC; tested species: rat
Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1.
Wang L, Kloc M, Maher E, Erisir A, Maffei A
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 135 421 IHC; tested species: rat
Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivo.
Dhar M, Hantman AW, Nishiyama H
Scientific reports (2018) 81: 17561. 135 421 IHC; tested species: mouse
Assembly of Excitatory Synapses in the Absence of Glutamatergic Neurotransmission.
Sando R, Bushong E, Zhu Y, Huang M, Considine C, Phan S, Ju S, Uytiepo M, Ellisman M, Maximov A
Neuron (2017) 942: 312-321.e3. 135 421 IHC; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 421 WB, UPTAKE; tested species: rat
WB
ICC
IHC
IHC-P
IHC-P
IHC-G
IHC-G
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
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