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Neuroligin 1/2/3/4 - 129 211

A postsynaptic cell adhesion molecule localized to postsynaptic densities
Monoclonal mouse purified IgG
Cat. No.: 129 211
Amount: 100 µg
Price: $415.00
Cat. No. 129 211 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: not recommended
IHC-P/FFPE: not tested yet
Clone 87H9
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 710 to 824 from mouse Neuroligin3 (UniProt Id: Q8BYM5)
Epitop Epitop: AA 608 to 722 from mouse Neuroligin3 (UniProt Id: Q8BYM5)
Reactivity Reacts with: rat (Q62765, Q62888, Q62889, ), mouse (Q99K10, Q69ZK9, Q8BYM5, B0F2B4).
Other species not tested yet.
Specificity WB: Recognizes neuroligin 2 and 3.
ICC: Stains predominantly neuroligin 2 in cultured hippocampus neurons. Detects transiently expressed GFP fusion proteins comprising the cytoplasmic tails of neuroligins 1-4. K.O.
Matching control protein/peptide 129-1P
Data sheet 129_211.pdf

References for Neuroligin 1/2/3/4 - 129 211

Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters.
Ye R, Quinlan MA, Iwamoto H, Wu HH, Green NH, Jetter CS, McMahon DG, Veestra-VanderWeele J, Levitt P, Blakely RD
Frontiers in synaptic neuroscience (2015) 7: 20. 129 211 WB; tested species: mouse
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 129 211 EM; tested species: rat
Cat. No.: 129 211
Quantity: 100 µg
Price: $415.00
Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters.
Ye R, Quinlan MA, Iwamoto H, Wu HH, Green NH, Jetter CS, McMahon DG, Veestra-VanderWeele J, Levitt P, Blakely RD
Frontiers in synaptic neuroscience (2015) 7: 20. 129 211 WB; tested species: mouse
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 129 211 EM; tested species: rat
Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin 1 and neuroligin 3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin 2 is found exclusively on inhibitory synapses.
Mutations in neuroligin 3 and neuroligin 4 have been implicated with a rare, heritable form of autism.