|Cat. No. 129 211||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery
IHC: not recommended
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 710 to 824 from mouse Neuroligin3 (UniProt Id: Q8BYM5)|
Epitop: AA 608 to 722 from mouse Neuroligin3 (UniProt Id: Q8BYM5)
Reacts with: rat (Q62765, Q62888, Q62889, ), mouse (Q99K10, Q69ZK9, Q8BYM5, B0F2B4).
Other species not tested yet.
WB: Recognizes neuroligin 2 and 3.
ICC: Stains predominantly neuroligin 2 in cultured hippocampus neurons. Detects transiently expressed GFP fusion proteins comprising the cytoplasmic tails of neuroligins 1-4. K.O.
|Matching control protein/peptide||129-1P|
Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin 1 and neuroligin 3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin 2 is found exclusively on inhibitory synapses.
Mutations in neuroligin 3 and neuroligin 4 have been implicated with a rare, heritable form of autism.