Synaptic Systems

Cat. No.: 124 012

Amount: 200 µl

Price: $355.00

Parvalbumin-producing striatal interneurons receive excitatory inputs onto proximal dendrites from the motor thalamus in male mice.
Nakano Y, Karube F, Hirai Y, Kobayashi K, Hioki H, Okamoto S, Kameda H, Fujiyama F
Journal of neuroscience research (2018) : . 124 012 IHC; tested species: mouse

WB

Background

PSD 95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.

1 ) Fujita A et al.,
2 ) Gundelfinger ED et al.,
3 ) Irie M et al.,
4 ) Chow RH et al.,
5 ) Kornau HC et al.,

Protocols

WB

IP

Standard protocol

OTHER

Preabsorbtion

Other products for this target

Cat. No. 124 012	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to PDZ-domain of mouse PSD95 (UniProt Id: Q62108)
Reactivity	Reacts with: rat (P31016), mouse (Q62108). Other species not tested yet.
Specificity	Specific for PSD 95 with weak cross-reactivity to SAP 102 K.O. validated
Matching control protein/peptide	124-01P
Data sheet	124_012.pdf

PSD95 antibody PDZ domain - 124 012 K.O.

References for PSD95 - 124 012