PSD95 antibody PDZ domain - 124 009 K.O.

PSD95 is a component of postsynaptic densities in central synapses

Chicken monoclonal recombinant IgY

Cat. No.: 124 009

Amount: 50 µg

Price: $420.00

Cat. No. 124 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	Ch108E10
Subtype	IgY (λ light chain)
Immunogen	Recombinant protein corresponding to PDZ-domain of mouse PSD95 (UniProt Id: Q62108)
Reactivity	Reacts with: rat (P31016), mouse (Q62108), chicken, human (P78352). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	124-01P
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 108E10. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells. IHC-G: The following fixatives are possible: 3% glyoxal, 9% glyoxal.
Data sheet	Datasheet 124_009

Cat. No.: 124 009

Amount: 50 µg

Price: $420.00

Indirect immunostaining of rat hippocampus neurons for PSD95 (magenta), MAP2 (cyan) and Synapsin1 (yellow)

ICC

IHC-G

Background

PSD95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K⁺ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD95 might be specialized for distinct functions. Read more: PSD95-antibody

1 ) Banker G et al., J Cell Biol (1974)
2 ) Hunt CA et al., J Neurosci (1996)
3 ) Stathakis DG et al., Genomics (1997)

Protocols

ICC

Fixed Cells

IHC-G

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