Synaptic Systems

Cat. No.: 124 008

Amount: 50 µg

Price: $425.00

Cat. No. 124 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet IHC-G: 1 : 500 (see remarks) gallery
Clone	Rb108E10
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to PDZ-domain of mouse PSD95 (UniProt Id: Q62108)
Reactivity	Reacts with: rat (P31016), mouse (Q62108), chicken, human (P78352). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	124-01P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 108E10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
Data sheet	124_008.pdf