Synaptic Systems

Cat. No.: 124 008

Amount: 50 µg

Price: $420.00

Cat. No. 124 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	Rb108E10
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to PDZ-domain of mouse PSD95 (UniProt Id: Q62108)
Reactivity	Reacts with: rat (P31016), mouse (Q62108), chicken, human (P78352). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	124-01P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 108E10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
Data sheet	124_008.pdf

References for PSD95 - 124 008

Roles of glutamate receptors in a novel in vitro model of early, comorbid cerebrovascular, and Alzheimer's diseases.
Simões-Pires EN, Ferreira ST, Linden R
Journal of neurochemistry (2020) : . 124 008 WB, IHC; tested species: rat

Regulation of synaptic connectivity in schizophrenia spectrum by mutual neuron-microglia interaction.
Breitmeyer R, Vogel S, Heider J, Hartmann SM, Wüst R, Keller AL, Binner A, Fitzgerald JC, Fallgatter AJ, Volkmer H
Communications biology (2023) 61: 472. 124 008 ICC; tested species: human

EF1α-associated protein complexes affect dendritic spine plasticity by regulating microglial phagocytosis in Fmr1 knock-out mice.
Su P, Yan S, Chen K, Huang L, Wang L, Lee FHF, Zhou H, Lai TKY, Jiang A, Samsom J, Wong AHC, et al.
Molecular psychiatry (2024) : . 124 008 IHC; tested species: mouse

Immunohistochemical characterization of bipolar cells in four distantly related avian species.
Balaji V, Haverkamp S, Seth PK, Günther A, Mendoza E, Schmidt J, Herrmann M, Pfeiffer LL, Němec P, Scharff C, Mouritsen H, et al.
The Journal of comparative neurology (2023) 5314: 561-581. 124 008 IHC

Cat. No.: 124 008

Amount: 50 µg

Price: $420.00

ICC

IHC-G

Background

PSD 95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.

1 ) Fujita A et al.,
2 ) Gundelfinger ED et al.,
3 ) Irie M et al.,
4 ) Chow RH et al.,
5 ) Kornau HC et al.,

Protocols

ICC

IHC-G

OTHER

Preabsorbtion

Other products for this target

PSD95 antibody PDZ domain - 124 008 K.O.

References for PSD95 - 124 008