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Home > Products > 307 011

Zyxin antibody - 307 011

Zyxin is involved in the regulation of actin dynamics
Mouse monoclonal purified IgG
Cat. No.: 307 011
Amount: 100 µg
Price: $415.00
Cat. No. 307 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 2000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 164D4
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 572 from human Zyxin (UniProt Id: Q15942)
Epitop AA 352 to 357 from human Zyxin (UniProt Id: Q15942)
Reactivity Reacts with: human (Q15942), rat, mouse (Q62523), hamster.
Other species not tested yet.
Data sheet 307_011.pdf

References for Zyxin - 307 011

Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1).
Endres M, Kneitz S, Orth MF, Perera RK, Zernecke A, Butt E
Oncotarget (2016) 739: 64244-64259. 307 011 WB
mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes.
Vollenbröker B, George B, Wolfgart M, Saleem MA, Pavenstädt H, Weide T
American journal of physiology. Renal physiology (2009) 2962: F418-26. 307 011 WB, ICC
Targeted disruption of the mouse Lipoma Preferred Partner gene.
Vervenne HB, Crombez KR, Delvaux EL, Janssens V, Van de Ven WJ, Petit MM
Biochemical and biophysical research communications (2009) 3792: 368-73. 307 011 WB
Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis.
Mori M, Nakagami H, Koibuchi N, Miura K, Takami Y, Koriyama H, Hayashi H, Sabe H, Mochizuki N, Morishita R, Kaneda Y, et al.
Molecular biology of the cell (2009) 2013: 3115-24. 307 011 WB, ICC
TES is a novel focal adhesion protein with a role in cell spreading.
Coutts AS, MacKenzie E, Griffith E, Black DM
Journal of cell science (2003) 116Pt 5: 897-906. 307 011 WB, ICC
The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions.
Garvalov BK, Higgins TE, Sutherland JD, Zettl M, Scaplehorn N, Köcher T, Piddini E, Griffiths G, Way M
The Journal of cell biology (2003) 1611: 33-9. 307 011 WB, ICC
Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Rottner K, Krause M, Gimona M, Small JV, Wehland J
Molecular biology of the cell (2001) 1210: 3103-13. 307 011 WB, ICC
GSK3 and lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration.
Dobson L, Barrell WB, Seraj Z, Lynham S, Wu SY, Krause M, Liu KJ
Cell reports (2023) 429: 113030. 307 011 ICC; tested species: mouse
Cell shape-dependent early responses of fibroblasts to cyclic strain.
Gadhari N, Charnley M, Marelli M, Brugger J, Chiquet M
Biochimica et biophysica acta (2013) 183312: 3415-3425. 307 011 ICC; tested species: mouse
Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.
Delaroche D, Cantrelle FX, Subra F, Van Heijenoort C, Guittet E, Jiao CY, Blanchoin L, Chassaing G, Lavielle S, Auclair C, Sagan S, et al.
The Journal of biological chemistry (2010) 28510: 7712-21. 307 011 ICC
Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis.
Low SH, Mukhina S, Srinivas V, Ng CZ, Murata-Hori M
Experimental cell research (2010) 31612: 1925-34. 307 011 ICC
mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes.
Vollenbröker B, George B, Wolfgart M, Saleem MA, Pavenstädt H, Weide T
American journal of physiology. Renal physiology (2009) 2962: F418-26. 307 011 WB, ICC
Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis.
Mori M, Nakagami H, Koibuchi N, Miura K, Takami Y, Koriyama H, Hayashi H, Sabe H, Mochizuki N, Morishita R, Kaneda Y, et al.
Molecular biology of the cell (2009) 2013: 3115-24. 307 011 WB, ICC
Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals.
Guo WH, Wang YL
Molecular biology of the cell (2007) 1811: 4519-27. 307 011 ICC
TES is a novel focal adhesion protein with a role in cell spreading.
Coutts AS, MacKenzie E, Griffith E, Black DM
Journal of cell science (2003) 116Pt 5: 897-906. 307 011 WB, ICC
The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions.
Garvalov BK, Higgins TE, Sutherland JD, Zettl M, Scaplehorn N, Köcher T, Piddini E, Griffiths G, Way M
The Journal of cell biology (2003) 1611: 33-9. 307 011 WB, ICC
Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Rottner K, Krause M, Gimona M, Small JV, Wehland J
Molecular biology of the cell (2001) 1210: 3103-13. 307 011 WB, ICC
Cat. No.: 307 011
Amount: 100 µg
Price: $415.00
Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1).
Endres M, Kneitz S, Orth MF, Perera RK, Zernecke A, Butt E
Oncotarget (2016) 739: 64244-64259. 307 011 WB
mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes.
Vollenbröker B, George B, Wolfgart M, Saleem MA, Pavenstädt H, Weide T
American journal of physiology. Renal physiology (2009) 2962: F418-26. 307 011 WB, ICC
Targeted disruption of the mouse Lipoma Preferred Partner gene.
Vervenne HB, Crombez KR, Delvaux EL, Janssens V, Van de Ven WJ, Petit MM
Biochemical and biophysical research communications (2009) 3792: 368-73. 307 011 WB
Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis.
Mori M, Nakagami H, Koibuchi N, Miura K, Takami Y, Koriyama H, Hayashi H, Sabe H, Mochizuki N, Morishita R, Kaneda Y, et al.
Molecular biology of the cell (2009) 2013: 3115-24. 307 011 WB, ICC
TES is a novel focal adhesion protein with a role in cell spreading.
Coutts AS, MacKenzie E, Griffith E, Black DM
Journal of cell science (2003) 116Pt 5: 897-906. 307 011 WB, ICC
The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions.
Garvalov BK, Higgins TE, Sutherland JD, Zettl M, Scaplehorn N, Köcher T, Piddini E, Griffiths G, Way M
The Journal of cell biology (2003) 1611: 33-9. 307 011 WB, ICC
Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Rottner K, Krause M, Gimona M, Small JV, Wehland J
Molecular biology of the cell (2001) 1210: 3103-13. 307 011 WB, ICC
GSK3 and lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration.
Dobson L, Barrell WB, Seraj Z, Lynham S, Wu SY, Krause M, Liu KJ
Cell reports (2023) 429: 113030. 307 011 ICC; tested species: mouse
Cell shape-dependent early responses of fibroblasts to cyclic strain.
Gadhari N, Charnley M, Marelli M, Brugger J, Chiquet M
Biochimica et biophysica acta (2013) 183312: 3415-3425. 307 011 ICC; tested species: mouse
Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.
Delaroche D, Cantrelle FX, Subra F, Van Heijenoort C, Guittet E, Jiao CY, Blanchoin L, Chassaing G, Lavielle S, Auclair C, Sagan S, et al.
The Journal of biological chemistry (2010) 28510: 7712-21. 307 011 ICC
Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis.
Low SH, Mukhina S, Srinivas V, Ng CZ, Murata-Hori M
Experimental cell research (2010) 31612: 1925-34. 307 011 ICC
mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes.
Vollenbröker B, George B, Wolfgart M, Saleem MA, Pavenstädt H, Weide T
American journal of physiology. Renal physiology (2009) 2962: F418-26. 307 011 WB, ICC
Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis.
Mori M, Nakagami H, Koibuchi N, Miura K, Takami Y, Koriyama H, Hayashi H, Sabe H, Mochizuki N, Morishita R, Kaneda Y, et al.
Molecular biology of the cell (2009) 2013: 3115-24. 307 011 WB, ICC
Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals.
Guo WH, Wang YL
Molecular biology of the cell (2007) 1811: 4519-27. 307 011 ICC
TES is a novel focal adhesion protein with a role in cell spreading.
Coutts AS, MacKenzie E, Griffith E, Black DM
Journal of cell science (2003) 116Pt 5: 897-906. 307 011 WB, ICC
The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions.
Garvalov BK, Higgins TE, Sutherland JD, Zettl M, Scaplehorn N, Köcher T, Piddini E, Griffiths G, Way M
The Journal of cell biology (2003) 1611: 33-9. 307 011 WB, ICC
Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Rottner K, Krause M, Gimona M, Small JV, Wehland J
Molecular biology of the cell (2001) 1210: 3103-13. 307 011 WB, ICC
WB
ICC
Background
Cell migration requires the formation of motile organelles like lamellipodia and the adhesion of the cell to the extracellular substrate. These specialized sites, termed focal adhesions, link the extracellular matrix to the actin cytoskeleton.
The LIM domain protein zyxin probably plays a key role in the regulation of actin dynamics at focal adhesions, along stress fibers and at cell-cell contacts.
Zyxin contains three C-terminal LIM-domains. The LIM domain consists of a conserved double zinc finger protein module which also occurs in transcription factors. It mediates the interaction with a wide variety of other proteins including other LIM domain proteins, kinases, transcription factors and cytoskeleton associated proteins.