Zyg11BL antibody - 335 003

Zyg11BL is a putative substrate recognition subunit of CBCs

Rabbit polyclonal purified antibody

Cat. No.: 335 003

Amount: 50 µg

Price: $375.00

Cat. No. 335 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 767 to 779 from mouse Zyg11BL (UniProt Id: Q3UFS0)
Reactivity	Reacts with: rat, mouse (Q3UFS0). Other species not tested yet.
Data sheet	335_003.pdf

Cat. No.: 335 003

Amount: 50 µg

Price: $375.00

Background

Mammalian Zyg11BL, a Zer 1 homolog, shows high levels of expression in skeletal muscle and the testes and is also clearly detectable in brain. It is believed to serve as a possible variable substrate recognition subunit (SRS) that confers substrate specificity to CBCs. CBCs are composed of the CUL 2, elongin B and C, Rbx 1/Roc 1 and one SRS.

1 ) White EA et al., Proc. Natl. Acad. Sci. U.S.A. (2012)
2 ) Mahrour N et al., J. Biol. Chem. (2008)
3 ) Vasudevan S et al., EMBO Rep. (2007)
4 ) Féral C et al., Mol. Hum. Reprod. (2001)

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