Synaptic Systems

Cat. No.: 166 103

Amount: 50 µg

Price: $380.00

ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 166 103 WB; KD verified; tested species: mouse

WB

ICC

IHC-P

Background

The essential micronutrient zinc (Zn²⁺) plays an important role in many biological processes like growth, development, and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn²⁺ bound water is involved in catalytic reactions. As a charged ion Zn²⁺ cannot cross biological membranes by simple diffusion and must be transported by specialized transport mechanisms. Two families of Zn²⁺ transporters, SLC30 (ZnT, Zn²⁺ transporter) and SLC39 (ZIP, Zinc (Zn²⁺)-Iron (Fe²⁺) permease), function in opposite directions to maintain cellular Zn²⁺ homeostasis (1).
Ten Zn²⁺ transporter proteins ZnT1-10 have been described. All of them contain several transmembrane domains and a histidine rich intracellular loop (2).
ZnT1 is abundantly expressed and has been suggested to be responsible for the efflux of Zn²⁺ from cells. It is upregulated by high oral Zn²⁺ doses. ZnT1 plays an essential role in maintaining neuronal Zn²⁺ balance, which is critical for synaptic signalling, neuroprotection, and the prevention of Zn²⁺ toxicity. Dysregulation of ZnT1 in neural tissues has been linked to neurodegenerative conditions and cognitive impairments (3). ZnT1 is involved in regulating Zn²⁺ levels within β-cells, which is essential for insulin storage and secretion. Proper ZnT1 function ensures optimal pancreatic endocrine activity and supports glucose homeostasis (4).

1 ) Huang L et al., Mol Aspects Med (2013)
2 ) Liuzzi JP et al., Annu Rev Nutr (2004)
3 ) Baltaci AK et al., Neurochem Res (2018)
4 ) Zhong ML et al., Histochem Cell Biol (2012)

Protocols

WB

ICC

IHC-P

Cat. No. 166 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 494 to 507 from rat ZnT1 (UniProt Id: Q62720)
Reactivity	Reacts with: rat (Q62720), mouse (Q60738). Other species not tested yet.
Specificity	K.D. validated PubMed: 34871934
Data sheet	166_103.pdf

ZnT1 antibody - 166 103 K.D.

References for ZnT1 - 166 103