ZnF365 antibody - 323 003

ZnF365 is a putative transcription factor

Rabbit polyclonal purified antibody

Cat. No.: 323 003

Amount: 50 µg

Price: $375.00

Cat. No. 323 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 363 to 410 from mouse ZnF365 (UniProt Id: Q8BG89)
Reactivity	Reacts with: rat (Q5PQS2), mouse (Q8BG89). Other species not tested yet.
Data sheet	323_003.pdf

Cat. No.: 323 003

Amount: 50 µg

Price: $375.00

ICC

Background

The Zn finger protein 365, also referred to as Talanin or DBZ, is a putative transcription factor. Mutations in the coding sequence have been associated with several diseases.
It has also been shown to interact with DISC1, a protein involved in schizophrenia.

1 ) Lindström S et al., Nat. Genet. (2011)
2 ) Haritunians T et al., Gut (2011)
3 ) Hattori T et al., Mol. Psychiatry (2007)
4 ) Gianfrancesco F et al., Gene (2004)

Protocols

ICC